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Showing: 1501 - 1550 of 4441 results
  1. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pFH113

    Plasmid
    #128210
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Depositor
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pSV40-GUS-P2A-Hygro

    Plasmid
    #106480
    Purpose
    Expression of GUS and Hygromycin resistance gene from SV40 promoter
    Depositor
    Inserts
    beta-glucuronidase
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pDOE20 mC VN

    Plasmid
    #124246
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.
    Depositor
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSW725 - pUFO>>

    Plasmid
    #116000
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Insert
    pUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pJRA103

    Plasmid
    #209336
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.
    Depositor
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pYPQ230-RV

    Plasmid
    #188544
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
    Depositor
    Insert
    LbCas12a-RV
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pUM31dao

    Plasmid
    #87242
    Purpose
    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants
    Depositor
    Insert
    D-amino oxidase
    Expression
    Plant
    Promoter
    plastid rrn
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1501 - 1550 of 4441 results