4,307 results

1. pEGB Pnos:Luciferase:Tnos (GB0112)

   Plasmid

   #68217

   Purpose

   Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pN_35S/mApple

   Plasmid

   #132566

   Purpose

   mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

   Insert

   mApple
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM100

   Plasmid

   #127225

   Purpose

   AtU6::sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDIRECT_25I

   Plasmid

   #91146

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

   Plasmid

   #160616

   Purpose

   TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEE390

   Plasmid

   #149280

   Purpose

   T-DNA encoding TRV2 with gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB4356

   Plasmid

   #187813

   Purpose

   Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and SpATF1-2.

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   Moth Pheromone pathway
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC-1-CmYLCV-6

   Plasmid

   #197699

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   CmYLCV promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-6-FLuc-9

    Plasmid

    #197737

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-6-RLuc-9

    Plasmid

    #197739

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Renilla luciferase (tomato optimized)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-9-AtHSP-11

    Plasmid

    #197760

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtHSP terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. cp-DdCBE G1333-N

    Plasmid

    #174556

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1333-N
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSi

    Plasmid

    #67884

    Purpose

    Derivative of pSiM24 plant expression vector without the M24 promoter

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFGC5941-PacI-ath-STTM173

    Plasmid

    #84101

    Purpose

    target miRNA173 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM173
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCBLggPro

    Plasmid

    #199736

    Purpose

    Golden Gate entry vector for promoter cloning

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGB3_omega2

    Plasmid

    #68239

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFH58

    Plasmid

    #128184

    Purpose

    Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P223

    Plasmid

    #139720

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_A'_pLHB1B1:NnCPH:tSlH4
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtLHB1B1

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGB1_omega1R

    Plasmid

    #68236

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192396

    Purpose

    To act as a 2-input state of the AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192391

    Purpose

    To act as a 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAM-35s-ABI2-YFPn-1

    Plasmid

    #102383

    Purpose

    split YFP. Plant expression of ABI2-YFPn-1

    Depositor

    Silke Robatzek

    Insert

    ABI2 (ABI2 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFGC5941-PacI-ath-STTM168

    Plasmid

    #84093

    Purpose

    target miRNA168 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM168
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB105-MpTubulin

    Plasmid

    #100588

    Purpose

    Binary vector for expression of Citrine-fused MpTubulin beta 3 (Citrine-MpTubulin) to visualize microtubules in plants

    Depositor

    Yutaka Kodama

    Article

    Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

    Insert

    Citrine-MpTubulin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lvl0 ACT7p

    Plasmid

    #153371

    Purpose

    Plant promoter

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    ACT7 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ141-ZmUbi-RZ-Aa3.8

    Plasmid

    #136376

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB14-LYX033

    Plasmid

    #126251

    Purpose

    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.

    Depositor

    Silin Zhong

    Insert

    Niben101Scf06112g01006.1
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pZYIntExc

    Plasmid

    #139673

    Purpose

    Expression cassette for phiC31 Excisionase in T-DNA vector

    Depositor

    James Birchler

    Article

    Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

    Insert

    phiC31 Excisionase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGMV-gSWEET4

    Plasmid

    #112796

    Purpose

    Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

    Depositor

    Humberto Prieto

    Article

    Prieto et al In: Cantu D., Walker M. (eds) The Grape

    Insert

    gRNA pair targeting VviSWEET4
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMAP L2Γ

    Plasmid

    #153358

    Purpose

    Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCBL101gg

    Plasmid

    #199735

    Purpose

    Golden Gate recipient vector for transgene overexpression

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    sacB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCBLggCDS

    Plasmid

    #199737

    Purpose

    Golden Gate entry vector for gene of interest cloning

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCBLggTerm

    Plasmid

    #199738

    Purpose

    Golden Gate entry vector for terminator cloning

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGWB452

    Plasmid

    #74834

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMpGWB121

    Plasmid

    #68575

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFH51

    Plasmid

    #128213

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFH23

    Plasmid

    #128180

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFH53

    Plasmid

    #128182

    Purpose

    Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMpGWB206

    Plasmid

    #68597

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge