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8,265 results

Showing: 701 - 750 of 8265 results

  6. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

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  16. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-Aga1P
    Tags
    V5 tag
    Expression
    Yeast
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73151-plasmid-map-sequence-id-134538

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  17. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185875-plasmid-map-sequence-id-369502

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  22. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  23. ZJOM160

    Plasmid
    #133670
    Purpose
    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-mTagBFP2-SKL
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133670-plasmid-map-sequence-id-267532

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  26. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  29. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  33. pGBW-m4046230

    Plasmid
    #145454
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145454-plasmid-map-sequence-id-300252

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  35. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Benjamin Glick
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Tags
    msYFP2
    Expression
    Yeast
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218969-plasmid-map-sequence-id-445656

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  37. pMM0641

    Plasmid
    #129006
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129006-plasmid-map-sequence-id-259445

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  38. pGBW-m4046376

    Plasmid
    #145481
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145481-plasmid-map-sequence-id-307563

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  39. pGBW-m4046560

    Plasmid
    #145471
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145471-plasmid-map-sequence-id-298269

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Showing: 701 - 750 of 8265 results