7,933 results
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Plasmid#103139PurposeExpresses Csy4-NLS under TEF promoterDepositorInsertCsy4
UseTagsNLSExpressionYeastMutationPromoterTEFAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMH124-VP16-CIB1
Plasmid#40615PurposeExpresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2DepositorInsertVP16-CIB1
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXZX501
Plasmid#169455PurposepRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassetteDepositorInsertgRNA for MATa
UseTagsExpressionYeastMutationPromoterSNR52Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
UseTagsHA tagExpressionYeastMutationPromoterpTEF1Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPDA1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV1326
Plasmid#111435PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS255
Plasmid#12382DepositorInsertthymidine kinase
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYZ301
Plasmid#102690PurposeS. pombe gRNA entry vector with Cas9 - Sp lys9 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIYC04
Plasmid#64741PurposeYeast expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNA0306
Plasmid#169452PurposepRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmidDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterTEF1pAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB768
Plasmid#60139PurposeGreen click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBG99
UseTagsExpressionYeastMutationPromoterMET17prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT0128
Plasmid#8729DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-bleMX6
Plasmid#19344DepositorInsertbleMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407A, I532APromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMAL358
Plasmid#169147PurposeEncodes OptoQ-AMP1DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
UseTagsExpressionBacterial and YeastMutationPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP420
Plasmid#26844DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0661
Plasmid#133476PurposeExpression in fission yeast from promoter pact1, integrated at ade6 locusDepositorInsertpAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXZX351
Plasmid#169454PurposepRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassetteDepositorInsertgRNA for MATα
UseTagsExpressionYeastMutationPromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS10-msGFPx3
Plasmid#105274PurposePlasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.DepositorInsertVps10 portion (PEP1 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL513
Plasmid#169153PurposeEncodes OptoQ-INVRT1DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
Plasmid#24549DepositorInsertpADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
UseTagsExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HyTkAX
Plasmid#73898Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P1
Plasmid#125034PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPGK1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Vph1-HaloTag
Plasmid#140149PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTagDepositorInsertVPH1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
UseTagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2196
Plasmid#67549PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3
Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsGFPMUT3ExpressionYeastMutationPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
p416 103Q GAL
Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP-SpHis5
Plasmid#44838PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selectionDepositorInsertyomTagBFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only