8,010 results
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Plasmid#166144PurposemRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-sGFP-Vrg4
Plasmid#160904PurposeExpresses GFP-Vrg4 in yeast cellsDepositorInsertVRG4
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Mouse, Budding Yeast)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB
Plasmid#14144DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM23
Plasmid#74643PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-YFP
Plasmid#29593DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS190
Plasmid#34977DepositorInsertePDZb
UseTagsExpressionYeastMutationPromoterADHAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-CaURA3
Plasmid#44877PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-P525L-YFP
Plasmid#29628DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationP525LPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNB2289
Plasmid#113396PurposeGalactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiaeDepositorInsertRed fluorescent protein, codon optimized for S. cerevisiae
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationPromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Synthetic, Budding Yeast, Human)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATP403red-hENT1
Plasmid#166027PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpTDH3Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)
Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB
Plasmid#14142DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNXRVb-HACre
Plasmid#78401Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
hygMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2225
Plasmid#67553PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA-his-HYB
Plasmid#64333Purposehis3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of his3 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXP422
Plasmid#26845DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-CaURA3
Plasmid#44873PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selectionDepositorInsertyoSuperfolderGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
PP244
Plasmid#78978PurposePromoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGKB
Plasmid#85077Purposeexpression in Pichia pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAPAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorInsertVph1-2GFP (VPH1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorInsertp1k-PEX1-2GFP (PEX1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8786
Plasmid#126920PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtIDepositorInsertXdCrtI
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179027PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only