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8,010 results

Showing: 1001 - 1040 of 8010 results
  1. pIMVAu1

    Plasmid
    #98301
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
    Depositor
    Insert
    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pNB2289

    Plasmid
    #113396
    Purpose
    Galactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiae
    Depositor
    Insert
    Red fluorescent protein, codon optimized for S. cerevisiae
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pRS426-HsFECH

    Plasmid
    #174524
    Purpose
    Expresses human ferrochelatase (FECH) in yeast
    Depositor
    Insert
    FECH with yeast HEM15 promoter and terminator (FECH Synthetic, Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Lacks original mitochondrial targeting sequence (…
    Promoter
    HEM15 (yeast, 257 bp)
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pCfB2225

    Plasmid
    #67553
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. ZJOM153

    Plasmid
    #133642
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.
    Depositor
    Insert
    Vph1-2GFP (VPH1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. ZJOM20

    Plasmid
    #133644
    Purpose
    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.
    Depositor
    Insert
    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1040 of 8010 results