8,057 results

1. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-LEU (V5 and HA)

   Plasmid

   #222486

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTU-DO-URA

   Plasmid

   #160004

   Purpose

   Low-copy expression vector for K. marxianus, uracil selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   n/a

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMTU-DO-HIS

   Plasmid

   #160005

   Purpose

   Low-copy expression vector for K. marxianus, histidine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTU-DO-LEU

   Plasmid

   #160006

   Purpose

   Low-copy expression vector for K. marxianus, leucine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML104-KanMx4

   Plasmid

   #83476

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   KanMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTPGI_dCas9

    Plasmid

    #64279

    Purpose

    encodes yeast-optimized dCas9 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    Yeast-optimized dCas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    D10A & H840A relative to S. pyogenes Cas9

    Promoter

    pTPGI

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEV-G1-Km

    Plasmid

    #46813

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #160389

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection

    Depositor

    John McCusker

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Cen/ars

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous ATG8 promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-6xGLY-3xFLAG-HIS3MX6

    Plasmid

    #20753

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-6xGLY-V5-HIS3MX6

    Plasmid

    #20777

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDLC201

    Plasmid

    #113388

    Purpose

    galactose-inducible Ty3

    Depositor

    Suzanne Sandmeyer

    Article

    Hansen et al Mol Cell Biol. 1988 Dec;8(12):5245-56.

    Insert

    Ty3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCUP1-GR

    Plasmid

    #1212

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    glucocorticoid receptor (Nr3c1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge