7,942 results

1. pYZ463

   Plasmid

   #187971

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ462

   Plasmid

   #187970

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEK19

   Plasmid

   #210578

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   BdBIP1pro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEK39

   Plasmid

   #210599

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
   

   Use

   Tags

   mRuby3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM112B

   Plasmid

   #210602

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2A/Bpro (divergent)

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

   Plasmid

   #58435

   Purpose

   Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)8-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRL_GI_PCB28

   Plasmid

   #98745

   Purpose

   For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

   Inserts

   heme oxygenase 1 (TED4 Mustard Weed)
   Phycocyanobilin:ferredoxin oxidoreductase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   ADH1m and PGK1

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. M4754 KanMX::HIS3 Disruptor Converter

    Plasmid

    #51684

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2988

    Plasmid

    #63638

    Purpose

    pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXRM15

    Plasmid

    #63150

    Purpose

    Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB2796

    Plasmid

    #63641

    Purpose

    pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p414GPD_Hsp82viiB

    Plasmid

    #61709

    Purpose

    Yeast plasmid with Hsp82 heterodimer construct.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiB (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    GPD1

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHOHO2_Hsp82viiA

    Plasmid

    #61708

    Purpose

    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiA (HSP82 Budding Yeast)
    

    Use

    Yeast integration vector targeting inserts to the…

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    TEF1

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_AXP_hrGFP

    Plasmid

    #84613

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPRyl_AXP

    Plasmid

    #84608

    Purpose

    Introduces DSB at AXP locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG425GAL-ccdB-EGFP

    Plasmid

    #14201

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge