8,350 results
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Plasmid#229994PurposeCarries AID* tag, empty sgRNA, and amdS markerDepositorInsertAID* - empty sgRNA - amdS
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303-LacZ
Plasmid#1169DepositorInsertLACZ
TagsGAL1:10 promoterExpressionYeastAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
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YEp112-CUP1-His-Smt3
Plasmid#99537PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2791
Plasmid#63654PurposepCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRW40
Plasmid#130973PurposeHyPr plasmid with nptII markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICXNH3
Plasmid#49020PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICXC3GH
Plasmid#49023PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLK
Plasmid#64186Purposeharbours marker genes LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
ExpressionYeastPromoterPGK1Available SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPM56
Plasmid#22855DepositorInserteroGFP & UPR-RFP
ExpressionYeastAvailable SinceJan. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0585
Plasmid#133472PurposeIntegration into fission yeast genome at ade6 locus with hphMX selection cassetteDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES-mtBFP
Plasmid#45055DepositorInsertmtBFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorInsertsSpCas9
PmCDA1
TagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWH
Plasmid#133894PurposeExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22HDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBKWH
Plasmid#133898PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-mCherry2Bx6
Plasmid#105273PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-CaURA3
Plasmid#44885PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selectionDepositorInsertyomKeima
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1
Plasmid#203163PurposeYeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXP320
Plasmid#26838DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO10-6SIM
Plasmid#190248PurposeExpression SUMO10-SIM6 in yeast cellsDepositorInsert10SUMO-6SIM
ExpressionYeastAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV115
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
p426 25Q GAL
Plasmid#1187DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
ExpressionYeastPromoterTetO7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXP741
Plasmid#46059DepositorTypeEmpty backboneExpressionYeastPromoterADH2Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
M2660 ura3::LYS2 Disruptor Converter
Plasmid#51678PurposeYeast marker swap plasmid for converting URA3 to LYS2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMF2
Plasmid#112819PurposeExpresses wild-type Rpb1 in yeast cellsDepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSSec7-mGFPx3
Plasmid#25449DepositorInsertSec7 (SEC7 Budding Yeast)
TagsmGFPx3ExpressionYeastMutationGFP contains A206K for monomerization.Available SinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet26
Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p426 72Q GPD
Plasmid#1183DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRG211
Plasmid#64519PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0753
Plasmid#133487PurposeExpression in fission yeast from promoter pnmt81 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneExpressionYeastPromoterpFUS1J2Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-URA3
Plasmid#224102PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with URA3 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-Kan
Plasmid#224090PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with Kan selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-SpHis5
Plasmid#224095PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with SpHis5 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yemiRFP670-URA3
Plasmid#224100PurposeYeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with URA3 selectionDepositorInsertyemiRFP670
TagsyemiRFP670ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMJ235: pRS426-His-MBP-GtFz1_Native
Plasmid#205254PurposeS. cerevisiae expression vector for GtFz1 native guideDepositorInsertGtFz1
Tags10xHis, MBPExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LPH2781 - Ub-K63R
Plasmid#32160DepositorExpressionYeastMutationK63RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
Tagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4787
Plasmid#106147PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only