8,016 results

1. pAD

   Plasmid

   #64747

   Purpose

   Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

   Depositor

   Jens Nielsen

   Article

   Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

   Insert

   ACC1 (ser659ala, ser1157ala)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG425GPD-EYFP-ccdB

   Plasmid

   #14346

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV106

   Plasmid

   #63196

   Purpose

   pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYES2.1-cerMET6

   Plasmid

   #107438

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET6 (MET6 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GAL FLAG46QDProGFP p306

   Plasmid

   #15832

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 46Q Delta Pro (HTT Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pAG426GAL-ccdB-EYFP

   Plasmid

   #14227

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pL-Sapphire

   Plasmid

   #176568

   Purpose

   Expression of Sapphire for auxotrophic selection in the absence of leucine

   Depositor

   Markus Ralser

   Insert

   yETSapphire
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGALSc104(A503V)

   Plasmid

   #1152

   Depositor

   Susan Lindquist

   Article

   Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

   Insert

   HSP104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A503V

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXNgate21-3HA

   Plasmid

   #105078

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046479

    Plasmid

    #145495

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDRf1-4CL5-DsRed (JBEI-15461)

    Plasmid

    #87935

    Purpose

    Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEN-L1-K2-L2

    Plasmid

    #80684

    Purpose

    Gateway Entry vector containing a temperature-sensitive mouse DHFR

    Depositor

    Nico Dissmeyer

    Article

    Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

    Inserts

    DHFR (Dhfr Mouse)
    DHFR (Dhfr Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    mouse DHFR with N-terminal Ubiquitin

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAC690-TEF

    Plasmid

    #160432

    Purpose

    Expresses eGFP under control of the yeast TEF2 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    S. cerevisiae TEF2 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S. cerevisiae TEF2

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. D771 leu2::LYS2 Disruptor Converter

    Plasmid

    #51677

    Purpose

    Yeast marker swap plasmid for converting LEU2 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV0471

    Plasmid

    #133490

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-URA-hPOLISc

    Plasmid

    #60819

    Purpose

    to express human Pol iota recoded to yeast codons in yeast

    Depositor

    Youri Pavlov

    Article

    Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

    Insert

    POLI (POLI Human)
    

    Use

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    optimized to yeast codon usage

    Promoter

    GAL1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MmSphk2

    Plasmid

    #118598

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV123

    Plasmid

    #63189

    Purpose

    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. N-GFP-Dcp2

    Plasmid

    #163585

    Purpose

    express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    DCP2 promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG416GAL-ccdB-mRuby3

    Plasmid

    #166139

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJJH1295

    Plasmid

    #36914

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    3x mCherry
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPM48

    Plasmid

    #22854

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    eroGFP & UPR-RFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pDA183

    Plasmid

    #74247

    Purpose

    pSTL1-dPSTRr(2,1)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pSTL1-dPSTRr(2,1)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLexAgtwy

    Plasmid

    #11345

    Depositor

    Guy Caldwell

    Insert

    gateway cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pRS416 Gal G294A YFP

    Plasmid

    #27448

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pRG235

    Plasmid

    #64533

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMetOYC-Dest

    Plasmid

    #105082

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV and mOST4

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHES875

    Plasmid

    #89192

    Purpose

    Expresses mCherry under control of GAL1 promoter.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pGAL1-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDRFLIP43

    Plasmid

    #65519

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDRFLIP39

    Plasmid

    #65517

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. plp

    Plasmid

    #22310

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Type

    Empty backbone

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMAL669

    Plasmid

    #169148

    Purpose

    Encodes OptoQ-AMP2

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMAL722

    Plasmid

    #169149

    Purpose

    Encodes OptoQ-AMP3

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMAL716

    Plasmid

    #169150

    Purpose

    Encodes OptoQ-AMP4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMZ283

    Plasmid

    #52224

    Purpose

    Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG425GPD-Cerulean-ccdB

    Plasmid

    #14418

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge