7,935 results
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Plasmid#125052PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertLAC4 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T2
Plasmid#125054PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertLAC4 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTY24
Plasmid#83794PurposeUseful for tagging yeast proteins with C-terminal DsRed tag; G418 selectionDepositorInsertDsRed
UseTagsDsRedExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK046
Plasmid#171318PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed.T1 fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP1975
Plasmid#159310PurposeYeast tagging vector to create gene fusion with yeast codon optimized mNeongreen under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM
Plasmid#64173Purposeharbours marker genes HIS3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046662
Plasmid#149221PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046690
Plasmid#149160PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046373
Plasmid#145555PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 WT
Plasmid#169261PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WTDepositorInsertNop4 WT (NOP4 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterGPDAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046228
Plasmid#145400PurposeYeast Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPSG830
Plasmid#158601PurposetNCS-ePTS1 (2micron)DepositorInsertpTDH3-tNCS-ePTS1
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 3-F GW
Plasmid#58559PurposeMulticopy vector with Hygromycin resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
LD211
Plasmid#69183PurposeIntegration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCherry
Plasmid#69857PurposeN-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-HA
Plasmid#14238DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
FRP1483
Plasmid#64511PurposeExpresses destabilized Ametrine in yeast.DepositorInsertAmetrine-ADH1-tail
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZCAN
Plasmid#129115PurposeZ. rouxii centromeric vector containing ClonNAT resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJA181
Plasmid#128539PurposeExpresses anti-Prunus dulcis scFv Ab+BirA tagDepositorInsertscFv Ab clone PD1F6 plus BirA tag
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FHyTkAX
Plasmid#73901Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179070PurposeVector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-preCOX4-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179052PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleusDepositorInsertRPL18Bp-SRM1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LD101
Plasmid#69160PurposeIntegration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg1-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K410N
Plasmid#87280PurposeEncodes Hsh155 K410N for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationK410NPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ACT_stable
Plasmid#127609Purposehigh-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3DepositorInsertStable ACT1 intron (Mut)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorUseTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306GPD-ccdB-HA
Plasmid#14236DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204-TC-Sec7-6xDsRed-M1
Plasmid#25448DepositorInsertSec7 (SEC7 Budding Yeast)
UseTags6xDsRed.M1ExpressionYeastMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRTagsExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT0103
Plasmid#8718DepositorInsertyEVenus
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYC52
Plasmid#63908PurposeRecipient integrative vector to construct CFP tagged fusionsDepositorTypeEmpty backboneUseTagsCFPExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GTH-r
Plasmid#81106PurposeGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Leu2 drive
Plasmid#89578PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locusDepositorInsertLeu2 gene drive
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-hSyntenin2b
Plasmid#89427PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGal4 ADExpressionYeastMutation*see belowPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 Q331K
Plasmid#27461DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlutamine 331 mutated to Lysine (Q331K)PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0771
Plasmid#133513PurposeExpression of mTagBFP2 tagged CHD domain to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
M4757 KanMX::TRP1 Disruptor Converter
Plasmid#51687PurposeYeast marker swap plasmid for converting KanMX3 to TRP1DepositorInsertTRP1 (TRP1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-URA
Plasmid#160025PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HIS
Plasmid#160005PurposeLow-copy expression vector for K. marxianus, histidine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-EGFP
Plasmid#14191DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-GaCYP722C(pYL777)
Plasmid#178290PurposeExpresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1DepositorInsertGaCYP722C
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXP414
Plasmid#26841DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2374
Plasmid#67534PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only