8,173 results
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Plasmid#188445PurposeCloning Vector and pDESTDepositorInsertccdB
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP8
Plasmid#166110PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5DepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_CycTER
Plasmid#61711PurposeYeast plasmid with Hsp82DepositorAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EYFP
Plasmid#14221DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ECFP-ccdB
Plasmid#14299DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046672
Plasmid#153918PurposeYeast expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG425GAL-ccdB-DsRed
Plasmid#14369DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
TagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
TagsL-IFP-F[2]ExpressionYeastPromoterGAL1Available SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKT-SCFP3A-HIS
Plasmid#64686PurposepKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.DepositorInsertSCFP3A
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet17
Plasmid#107453PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LD231
Plasmid#69193PurposeIntegration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg31-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LHP424-YCplac33-STE2-WT
Plasmid#32171DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM394
Plasmid#84204PurposeDisturb the expression of miR394 in riceDepositorInsertmiR394
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Bt δCOP
Plasmid#73871PurposeBos taurus Arcn1DepositorInsertRet2 Promoter- COPD
ExpressionYeastPromoterRet2 PromoterAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHLK
Plasmid#64182Purposeharbours marker genes HIS3, LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-TAP
Plasmid#14268DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
TagsHaloTagExpressionYeastPromoterEndogenousAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXP412
Plasmid#26840DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-STV1-iGFP
Plasmid#177111PurposeExpresses Stv1-iGFP in yeast cellsDepositorInsertSTV1 (STV1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R521H-YFP
Plasmid#29624DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYC44
Plasmid#63903PurposeInitial Integrative VectorDepositorTypeEmpty backboneTagsFRT::3'UTRcta::NAT::FRTExpressionYeastAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-Lam
Plasmid#133899PurposeNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
P413TEF-PYK1
Plasmid#34605DepositorInsertPYK1
ExpressionYeastPromoterTEFAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
BB3aN_14*
Plasmid#98548Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPtdh3Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
ExpressionYeastPromoterpFUS1J2Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP1432
Plasmid#64509PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAH1
Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7, TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-S-tag-hphMX4
Plasmid#20767DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHpgRNA50
Plasmid#160128PurposeTo express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-LSC2- HDV-TAMO
ExpressionYeastAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
TagsFLAG tagExpressionYeastPromoterGAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFS118
Plasmid#12378DepositorInsertura4, thymidine kinase
ExpressionYeastAvailable SinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXP314
Plasmid#26835DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPS2167
Plasmid#1365DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
POM42-mCitrine
Plasmid#69854PurposeN-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179077PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosolDepositorInsertTDH3p-Neon(gfp)
ExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTM116-D9
Plasmid#111232Purposeyeast expression vectorDepositorInsertccdB-cassette
ExpressionYeastAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD111
Plasmid#69165PurposeIntegration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-MYO1
Plasmid#51570PurposeConstitutively express myosin ring localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsMYO1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only