7,935 results
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Plasmid#178297PurposeExpresses VvCYP722C from V. vinifera in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VvCYP722C-TCYC1DepositorInsertVvCYP722C
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-GmCYP722C(pYL887)
Plasmid#178298PurposeExpresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1DepositorInsertGmCYP722C
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-FvCYP722C2(pYL878)
Plasmid#178299PurposeExpresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1DepositorInsertFvCYP722C2
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1515
Plasmid#62385PurposepFA6a Osm1-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationPromoterAdh1pAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-OsCYP711A3(pYL761)
Plasmid#178300PurposeExpresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1DepositorInsertOsCYP711A3
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046406
Plasmid#153880PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046463
Plasmid#145462PurposeYeast Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-NatMx
Plasmid#122903PurposeTRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
UseTagsExpressionYeastMutationPromoterLEU1prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarlet-T2A-mTurquoise2
Plasmid#118431PurposeProduces equimolar expression of mScarlet and mTq2DepositorInsertmScarlet-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM166
Plasmid#84197PurposeDisturb the expression of miR166 in riceDepositorInsertmiR166
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ECFP-ccdB
Plasmid#14298DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0662
Plasmid#133477PurposeExpression in fission yeast from promoter pact1, integrated at lys3 locusDepositorInsertpLys3(BstZ17I)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
UseTagsExpressionYeastMutationValine 163 changed to alaninePromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-EYFP-ccdB
Plasmid#14340DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2194
Plasmid#67545PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP122
Plasmid#26827DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
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YIp ADE2-NatMx
Plasmid#122918PurposeADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP266
Plasmid#159304PurposeYeast tagging vector to create gene fusion with yeast codon optimized mKate2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046173
Plasmid#149126PurposeYeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1-GW mVenus-T2A-mTurquoise2
Plasmid#118434PurposeProduces equimolar expression of mVenus and mTq2DepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK078
Plasmid#171350PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTxlnA ANIA_03613 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK077
Plasmid#171349PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTpenDE Pc21g21370 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK076
Plasmid#171348PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtif35 Pc22g19890 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK085
Plasmid#171357PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Leu] P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK053
Plasmid#171325PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertalcR ANIA_08978 transcriptional activator (no intron)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK090
Plasmid#171362PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert3'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK089
Plasmid#171361PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert5'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK083
Plasmid#171355PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu6 hom., P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK082
Plasmid#171354PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu3 hom., Tutp25, P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK081
Plasmid#171353PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTactA ANIA_06542 P. rubens terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK075
Plasmid#171347PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTamdS ANIA_08777 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only