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8,265 results

Showing: 2951 - 3000 of 8265 results

  1. pGBW-m4046418

    Plasmid
    #149029
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149029-plasmid-map-sequence-id-297818

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  3. pAAH2

    Plasmid
    #41826
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41826-plasmid-map-sequence-id-69077

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  7. THI VP64 pGAP T5

    Plasmid
    #161492
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161492-plasmid-map-sequence-id-326163

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  10. THI VP64 pGAP T1

    Plasmid
    #161488
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161488-plasmid-map-sequence-id-326159

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  12. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  23. pGBW-m4046437

    Plasmid
    #145415
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145415-plasmid-map-sequence-id-298868

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  27. pGBW-m4046431

    Plasmid
    #145526
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145526-plasmid-map-sequence-id-298267

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  29. pGBW-m4046476

    Plasmid
    #145512
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145512-plasmid-map-sequence-id-298324

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  31. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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  38. pAW8-aid-V5-Turg1:kanMX6

    Plasmid
    #169357
    Purpose
    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169357-plasmid-map-sequence-id-333200

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  40. pFA6a-link-mKO1-SpHis5

    Plasmid
    #44642
    Purpose
    Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    mKO1
    Expression
    Yeast
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44642-plasmid-map-sequence-id-61105

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  42. pCfB2192

    Plasmid
    #67539
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67539-plasmid-map-sequence-id-116407

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  45. pGBW-m4046640

    Plasmid
    #153906
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153906-plasmid-map-sequence-id-300446

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Showing: 2951 - 3000 of 8265 results