8,173 results
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Plasmid#14191DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only
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pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MtPT4
Plasmid#117096Purposeprotein expression in yeastDepositorInsertMtPT4
TagsHisExpressionYeastPromoterAOXAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
423Gal-FUS-YFP
Plasmid#29615DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G9-5FLAG-kanMX6
Plasmid#49181Purposegenomic G9-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneTagsG9-5FLAGExpressionYeastAvailable SinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBM5218
Plasmid#18796DepositorInsertTy5-HIS3AI
ExpressionYeastAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-Ub-R-HsACTB*-thymosinB-8His
Plasmid#111149PurposeExpresses arginylated human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationN-terminal arginylationPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB9337 (pgRNA_IV-1_NatMX)
Plasmid#161590PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
Plasmid#52689PurposeSame as 41022, with additional linkerDepositorInserttetO7-Pcyc1-3xFLAG+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-HA
Plasmid#14238DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
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Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec7-mScarlet2Ix6
Plasmid#140151PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6DepositorInsertSEC7
ExpressionYeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB-HA
Plasmid#14248DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
TagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneTagsmKate2ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2227
Plasmid#67536PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP414
Plasmid#26841DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGBT metaxin 2
Plasmid#12419DepositorInsertmetaxin 2 (Mtx2 Mouse)
ExpressionYeastAvailable SinceOct. 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-SpHis5
Plasmid#44857PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selectionDepositorInsertyoClover
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRS426-ACT_stable
Plasmid#127609Purposehigh-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3DepositorInsertStable ACT1 intron (Mut)
ExpressionYeastAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-URA
Plasmid#188922PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSCupGFP
Plasmid#1086DepositorInsertGFP
ExpressionYeastAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pH-eCFP
Plasmid#176555PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-eCFP
Plasmid#176556PurposeExpression of eCFP for auxotrophic selection in the absence of leucineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-eCFP
Plasmid#176557PurposeExpression of eCFP for auxotrophic selection in the absence of uracilDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA (405)
Plasmid#121417PurposeExpressing Dhh1-PADepositorInsertDhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
ExpressionYeastAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
M3499 ura3::ADE2 Disruptor Converter
Plasmid#51674PurposeYeast marker swap plasmid for converting URA3 to ADE2DepositorAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJZC595
Plasmid#62322PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationHas nuclease-inactivating mutations in the RuvC1 …PromoterTdh3 and pAdhAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-SpHis5
Plasmid#44843PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selectionDepositorInsertyomRuby
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1665
Plasmid#112039PurposeIntegrating plasmid for expression of ER localized mCherry at TRP1 locusDepositorInsertER-mCherry
ExpressionYeastPromoterTPIAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P81nmt1-3FLAG
Plasmid#19350DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pICXC3H
Plasmid#49022PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYC50
Plasmid#63906PurposeRecipient integrative vector to construct MYC tagged fusionsDepositorTypeEmpty backboneTagsMYCExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSK450
Plasmid#34552DepositorInsertMS2-CP-NLS
TagsmCherryExpressionYeastPromoterMet25Available SinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only