7,935 results
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Plasmid#73284PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046464
Plasmid#148970PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK049
Plasmid#171321PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertmCherry fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1-YFP
Plasmid#18685DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-SpHis5
Plasmid#44850PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selectionDepositorInsertyomWasabi
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
KAP95-FLU (113)
Plasmid#24045DepositorInsertKAP95 (KAP95 Budding Yeast)
UseTagsFLUExpressionYeastMutationPromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS316 GAL cluster
Plasmid#61925PurposeExpression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL6-msGFPx3
Plasmid#105254PurposePlasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.DepositorInsertApl6 portion (APL6 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB-EYFP
Plasmid#14217DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
UseTagsExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP-ccdB
Plasmid#14315DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationR201APromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCP5
Plasmid#35397DepositorTypeEmpty backboneUseReporterTagsExpressionYeastMutationPromoterGDP promoterAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-HSV-hphMX4
Plasmid#20776DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-His3MX6
Plasmid#69850PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-KanMx
Plasmid#122896PurposeADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS22H
Plasmid#133904PurposeExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mDsRed-SpHis5
Plasmid#44639PurposeYeast tagging vector to create gene fusion with mDsRed with SpHis5 selectionDepositorInsertmDsRed
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HIS
Plasmid#160016PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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M2371 his3::ADE2 Disruptor Converter
Plasmid#51671PurposeYeast marker swap plasmid for converting HIS3 to ADE2DepositorInsertADE2 (ADE2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterADE2Available sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EGFP
Plasmid#14190DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDS282
Plasmid#34983DepositorInsertcpPDZ–mCherry
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046179
Plasmid#149059PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG303GAL-EGFP-ccdB
Plasmid#14301DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4796
Plasmid#106131PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138I, T78HPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC824
Plasmid#20384DepositorInsertUAS cassette (FUS1)
UseTagsExpressionYeastMutationX=UbiY (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSEYC68-HA-TOR2 (pAS21)
Plasmid#39207DepositorInsertTOR2 (TOR2 Budding Yeast)
UseTagsHAExpressionYeastMutationPromoterGALAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXP214
Plasmid#26829DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
BiFC3
Plasmid#190048PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
UseTagsExpressionYeastMutationPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only