7,935 results

1. pJK370

   Plasmid

   #73595

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 2)
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Silent mutation in Arg299 codon.

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS405 VAC8-RFP

   Plasmid

   #166841

   Purpose

   Expresses Vac8-RFP in yeasts

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG426GPD-ccdB-EYFP

   Plasmid

   #14228

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-link-yoLSS-mKate2-SpHis5

   Plasmid

   #44848

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Desmin/pAS2-1

   Plasmid

   #97407

   Purpose

   Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   Desmin (DES Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pAG426GPD-ccdB-TAP

   Plasmid

   #14276

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIplac211-STV1-iGFP

   Plasmid

   #177111

   Purpose

   Expresses Stv1-iGFP in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   STV1 (STV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-bleMX6-P81nmt1-3FLAG

   Plasmid

   #19353

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   bleMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW8-aid-V5-Turg1:kanMX6

   Plasmid

   #169357

   Purpose

   C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   1XV5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR208IM1

    Plasmid

    #45351

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Insert

    UBR1 mutant
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D176E

    Promoter

    ADH

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Destination Vector

    Plasmid

    #125754

    Purpose

    Destination vector for assembling gRNA fragments in the second round of assembly.

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    pTDH3-GFPdropout-tTDH1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BB3aN_14*

    Plasmid

    #98548

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDSM-bc-Ptdh3-CAD-Ttdh1

    Plasmid

    #127727

    Purpose

    Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptdh3

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG413GPD-ccdB-DsRed

    Plasmid

    #14358

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pL59-Nat

    Plasmid

    #140465

    Purpose

    CRISPR/Cas9 engineering with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTCW008

    Plasmid

    #83526

    Purpose

    pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

    Insert

    pFUS1J2-mfalpha2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-FTKanAX

    Plasmid

    #73900

    Purpose

    fission yeast drug counter-selectable selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    TK-kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. D5 gene in PETCON vector

    Plasmid

    #87626

    Purpose

    expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

    Insert

    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
    

    Use

    Tags

    3x(G4S) Linker and cMyc tag on backbone

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRP1625

    Plasmid

    #64537

    Purpose

    Expresses LexA-ER-B42 in yeast cells

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Insert

    LexA-ER-B42
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHpgRNA50

    Plasmid

    #160128

    Purpose

    To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-LSC2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGBW-m4046418

    Plasmid

    #149029

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pGBW-m4046640

    Plasmid

    #153906

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pICE ADE2-URA3

    Plasmid

    #122886

    Purpose

    ADE2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDS191

    Plasmid

    #34978

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FRP2002

    Plasmid

    #159311

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTH650-RLuc/staFLuc

    Plasmid

    #29696

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 May 9. ():.

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p414TEF_Hsp82_CycTER

    Plasmid

    #61711

    Purpose

    Yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG426GPD-ccdB-ECFP

    Plasmid

    #14180

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGAD-GH-MEFV

    Plasmid

    #92106

    Purpose

    Yeast two hybrid

    Depositor

    Feng Shao

    Article

    Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

    Insert

    MEFV (MEFV Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG303GAL-ccdB-EYFP

    Plasmid

    #14205

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGWKS4

    Plasmid

    #139411

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179077

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCUP1-NMGR

    Plasmid

    #1211

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)
    

    Use

    Tags

    GR

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LD111

    Plasmid

    #69165

    Purpose

    Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg11-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB5935

    Plasmid

    #106128

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PRS415-Gal1pr-sctetR-FokI

    Plasmid

    #44756

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    sctetR-FokI (fokI Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1pr

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge