8,025 results

1. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB4132

   Plasmid

   #106127

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JME4393

   Plasmid

   #129657

   Purpose

   LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV0479

   Plasmid

   #133499

   Purpose

   Expression of sfGFP excluded from the nucleus with two NES signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ZJOM108

   Plasmid

   #133665

   Purpose

   Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV0543

   Plasmid

   #133493

   Purpose

   Expression of nucleo-cytoplasmic mCherry in P-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWKS12

   Plasmid

   #139419

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   Superfolder GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RPL25NLS-GFP (101)

   Plasmid

   #24038

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Promoter

   Available since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

9. YIplac211-iGFP-YPT31

   Plasmid

   #105263

   Purpose

   Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Ypt31 (YPT31 )
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Osa-pCAMBIA-1301-STTM159

    Plasmid

    #84195

    Purpose

    Disturb the expression of miR159 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR159
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pCri-15a

    Plasmid

    #61323

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS415-SOH1

    Plasmid

    #8622

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    SOH1 (SOH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNB813

    Plasmid

    #60161

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSK247

    Plasmid

    #131148

    Purpose

    dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSUP35, min pCYC1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVG48

    Plasmid

    #164706

    Purpose

    rtTA under the control of PGK1 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    PGK1pr - rtTA - CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJW1673

    Plasmid

    #112047

    Purpose

    CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    SCT1
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRPR1_c3gRNA_RPR1t

    Plasmid

    #64381

    Purpose

    encodes c3 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c3 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pML104-kanR1136

    Plasmid

    #162040

    Purpose

    Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    kanR1136 guide
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG416GPD-Cerulean-YPT7

    Plasmid

    #18847

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT7 (YPT7 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

21. PP363

    Plasmid

    #78984

    Purpose

    255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    rHGH
    IFNa2b
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRB2960

    Plasmid

    #8677

    Depositor

    David Botstein

    Article

    Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

    Insert

    GFP::TUB1 under GAL1 promoter (TUB1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

23. pFS177

    Plasmid

    #12380

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N81S and M84V

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNB330

    Plasmid

    #60147

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVP64

    Plasmid

    #22287

    Depositor

    Louise Prakash

    Article

    Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

    Insert

    REV1 (REV1 Budding Yeast)
    

    Use

    Tags

    4xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pI3-MTU-DO-HPH

    Plasmid

    #160207

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pI3-MTU-DO-LEU

    Plasmid

    #160176

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pI4-MTU-DO-LEU

    Plasmid

    #160029

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pI3-MTU-DO-HIS

    Plasmid

    #160021

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pI3-MTU-DO-URA

    Plasmid

    #160020

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMTU-DO-LEU

    Plasmid

    #160006

    Purpose

    Low-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGRE GFP

    Plasmid

    #1196

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    GFP w/ stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GFP w/ stop codon

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pADH1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p416TEF-SEC16

    Plasmid

    #102676

    Purpose

    The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

    Depositor

    Jens Nielsen

    Article

    Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

    Insert

    SEC16 (SEC16 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-bleMX6-P81nmt1-3FLAG

    Plasmid

    #19353

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    bleMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG426GPD-ccdB-EYFP

    Plasmid

    #14228

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVD_85

    Plasmid

    #149604

    Purpose

    (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    XylR
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-link-mKO1-SpHis5

    Plasmid

    #44642

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HsCERK

    Plasmid

    #118600

    Purpose

    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens ceramide kinase
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Feb. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge