8,043 results

1. 426Gal-FUS-267-526aa-YFP

   Plasmid

   #29605

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-267-526aa (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Contains FUS aa# 267-526

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pBK1

   Plasmid

   #1213

   Depositor

   Susan Lindquist

   Article

   Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

   Insert

   Hsc82::LEU2 (HSC82 Budding Yeast)
   

   Use

   Integration

   Tags

   Expression

   Yeast

   Mutation

   hsc82::LEU2 deletion select for plasmid using LE…

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA3

   Plasmid

   #46790

   Purpose

   To express ATPase inactive Fun30 in budding yeast

   Depositor

   Patrick Varga-Weisz

   Article

   Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

   Insert

   FUN30 (FUN30 Budding Yeast)
   

   Use

   Tags

   V5

   Expression

   Yeast

   Mutation

   ATP-binding site mutated replaced lysin 603 by ar…

   Promoter

   PGAL1

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pGBKT7-PRP43

   Plasmid

   #87596

   Purpose

   Encodes Y2H Gal4 DNA binding domain for Prp43

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   PRP43 (PRP43 Budding Yeast)
   

   Use

   Tags

   GAL4 BD and Myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG414GPD-ccdB-DsRed

   Plasmid

   #14360

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YIp TRP1-URA3

   Plasmid

   #122915

   Purpose

   TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pILGFPQ3

   Plasmid

   #83558

   Purpose

   Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL2

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDS247

   Plasmid

   #34967

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T406A, T407A, V416I, T–2S

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDS248

   Plasmid

   #34968

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T406A, T407A, V416I

   Promoter

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPS1890

    Plasmid

    #1362

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-ECFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pTSK241

    Plasmid

    #34550

    Depositor

    James McNally

    Article

    Karpova et al Science. 2008 Jan 25;319(5862):466-9.

    Insert

    Ace1 (CUP2 Budding Yeast)
    

    Use

    Integrative plasmid

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    CAP2

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046534

    Plasmid

    #145571

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. YIp204-P30-His-POL30

    Plasmid

    #99550

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046495

    Plasmid

    #148983

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pPTK008-3a-αMFΔ no Kex

    Plasmid

    #84967

    Purpose

    Type 3a, α-mating factor no Kex recognition site - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor no Kex recognition site secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG305GAL-ccdB-EYFP

    Plasmid

    #14209

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PSUC2-GFPantisense-CYC1t-pRS413

    Plasmid

    #64404

    Purpose

    SUC2 regulated expression of an antisense RNAi construct for GFP

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    GFPantisense
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUC2

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-PAmKate-KanMX6

    Plasmid

    #87028

    Purpose

    Tag S. pombe genes in C terminal with PAmKate

    Depositor

    Julien Berro

    Insert

    PAmKate
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPS832

    Plasmid

    #1357

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

21. pAG416GPD-Cerulean-CHC1

    Plasmid

    #18850

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    CHC1 (CHC1 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pGBW-m4046300

    Plasmid

    #149043

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGBW-m4046182

    Plasmid

    #149019

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pBW105

    Plasmid

    #111137

    Purpose

    For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

    Depositor

    Alexander Varshavsky

    Article

    Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

    Insert

    MLS-ratAANAT-3FLAG (Aanat Rat)
    

    Use

    Tags

    3x Flag

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA6a-his3-P3nmt1-3FLAG

    Plasmid

    #19339

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    his3 (his3 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS415-BUD32

    Plasmid

    #10991

    Depositor

    Francisco Malagon

    Insert

    BUD32 (BUD32 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAS2-1-Esf1

    Plasmid

    #99987

    Purpose

    Express yeast Esf1 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Esf1 (ESF1 Budding Yeast)
    

    Use

    Tags

    Y2H binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPTK011-3a-SA-αMFΔ

    Plasmid

    #84970

    Purpose

    Type 3a, Serum albumin followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Serum albumin followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPS1891

    Plasmid

    #1363

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

    Plasmid

    #50032

    Purpose

    Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA-HDEL

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p426 1-F GW

    Plasmid

    #58548

    Purpose

    pRS426 carrying Gateway cassette for a single insert

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCP5b

    Plasmid

    #37185

    Depositor

    Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDRF1-GW ymTurquoise2-T2A-mCherry

    Plasmid

    #118442

    Purpose

    Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. YIp ADE2-KanMx

    Plasmid

    #122917

    Purpose

    ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCTD26-S>A10-17

    Plasmid

    #112826

    Purpose

    pSMF2 with all S to A mutations in only repeats 10-17.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCTD26-S>A2-9

    Plasmid

    #112825

    Purpose

    pSMF2 with all S to A mutations in only repeats 2-9.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 2…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCTD26-S>A18-25

    Plasmid

    #112827

    Purpose

    pSMF2 with all S to A mutations in only repeats 18-25.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG413GAL-ccdB-ECFP

    Plasmid

    #14165

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCHFLAGGR

    Plasmid

    #1279

    Depositor

    Susan Lindquist

    Article

    Kimura et al Science 1995 Jun 2;268(5215):1362-5.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

40. pPTK019-4-tAOX1

    Plasmid

    #84988

    Purpose

    Type 4, tAOX, Alcohol oxidase 1 terminator

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Alcohol oxidase 1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge