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Plasmid#149048PurposeYeast Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046194
Plasmid#148979PurposeYeast Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046521
Plasmid#145473PurposeYeast Expression plasmid for SARS-CoV-2 nsp11DepositorInsertSARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXP812
Plasmid#46062DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP84
Plasmid#83542PurposeUsed to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.DepositorInsertSSA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSA1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB-TAP
Plasmid#14254DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046696
Plasmid#153882PurposeYeast expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXP731
Plasmid#46057DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH399-SUP45-S30P
Plasmid#29372DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationS30P mutant gene (corresponding to the sl23ts allā¦PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH375-SUP45-P174Q
Plasmid#29383DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationP174Q mutant gene including genomic sequence 1432ā¦PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
p41Hyg 3-F GW
Plasmid#58555PurposeCEN vector with Hygromycin resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2799
Plasmid#63658PurposepCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB-HA
Plasmid#14230DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICE TRP1-URA3
Plasmid#122894PurposeTRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-HphMx
Plasmid#122898PurposeLYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-KanMx
Plasmid#122899PurposeLYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-NatMx
Plasmid#122900PurposeLYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HphMx
Plasmid#122901PurposeTRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
12x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64393Purposeencodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
Plasmid#126739Purposeyeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2, acs1*, ald6
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, S629N (please see depositor commā¦PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-ECFP
Plasmid#14174DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Nup124-GFP-MTn
Plasmid#154365PurposeYeast expression of Nup124DepositorInsertNup124 (nup124 Fission Yeast)
UseTagsExpressionYeastMutationSee depositor comments belowPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2198
Plasmid#67554PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAL HA25QDProCFPp303
Plasmid#15578DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL HA103QDProCFPp303
Plasmid#15579DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ECFP-ccdB
Plasmid#14297DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pCfB2228
Plasmid#67540PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2992
Plasmid#63642PurposepCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG217
Plasmid#64531PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB3aK_14*
Plasmid#98529Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS34-Pleum
Plasmid#60334PurposeContains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG424GPD-ccdB-Cerulean
Plasmid#14392DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJZC548
Plasmid#62316PurposesgRNA with 1x PP7 for yeast cellsDepositorInsertsgRNA + 1x PP7
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS413-IVb
Plasmid#31459DepositorInsertlinker
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only