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8,017 results

Showing: 241 - 270 of 8017 results
  1. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCfB2197

    Plasmid
    #67551
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pYI2165

    Plasmid
    #228347
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression
    Depositor
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pFA6a-link-ymTq2-URA3

    Plasmid
    #168057
    Purpose
    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP
    Depositor
    Insert
    ymTurquoise2
    Use
    Tags
    ymTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pSH62-EBD

    Plasmid
    #49455
    Purpose
    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
    Depositor
    Insert
    Cre-EBD fusion
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1 promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. p414-TEF1p-Cas9-CYC1t

    Plasmid
    #43802
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter
    Depositor
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. ZJOM151

    Plasmid
    #133643
    Purpose
    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.
    Depositor
    Insert
    p1k-COX4-GFP (COX4 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pOGS539_haPD-1

    Plasmid
    #226716
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)
    Depositor
    Insert
    High affinity PD-1 microbody
    Use
    Tags
    HA tag and Mating factor alpha secretion signal
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pOGS539_rPD-L1nb

    Plasmid
    #226717
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)
    Depositor
    Insert
    Recombinant PD-L1 nanobody
    Use
    Tags
    HA tag, Histidine tag, and Mating factor alpha se…
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 270 of 8017 results