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Showing: 1 - 50 of 715 results

  4. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  5. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  10. DUSP7

    Plasmid
    #27976
    Depositor
    Igor Astsaturov
    Insert
    DUSP7 (DUSP7 Human)
    Use
    Lentiviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pETM33_Nsp7

    Plasmid
    #156476
    Purpose
    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156476-plasmid-map-sequence-id-307742

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  17. ∆DUSP7

    Plasmid
    #27978
    Depositor
    Igor Astsaturov
    Insert
    DUSP7 (DUSP7 Human)
    Use
    Lentiviral
    Tags
    HA and MYC
    Expression
    Mammalian
    Mutation
    C280S: mutation in the phosphatase domain
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

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  25. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  29. pKM263-USP7

    Plasmid
    #196365
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    USP7 catalytic domain
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only S207-E560
    Promoter
    T7
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196365-plasmid-map-sequence-id-386294

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  32. PRE-Hsp70BbCas9_1.2

    Plasmid
    #190796
    Purpose
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190796-plasmid-map-sequence-id-376571

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  38. MSP7-bio

    Plasmid
    #47735
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP7
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47735-plasmid-map-sequence-id-68747

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  40. pSP72 GSK3beta

    Plasmid
    #60125
    Purpose
    for transcription of GSK3beta
    Depositor
    Andrew McMahon
    Insert
    Glycogen synthase kinase 3 beta (Gsk3b Mouse)
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60125-plasmid-map-sequence-id-307046

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  42. PRE-Hsp70BbCas9_1.3

    Plasmid
    #190797
    Purpose
    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190797-plasmid-map-sequence-id-376572

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  43. MSP7.9-bio

    Plasmid
    #68514
    Purpose
    Expresses full-length P. vivax MSP7.9 (PVX_082655) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    MSP7.9
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    68514-plasmid-map-sequence-id-120622

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  44. MSP7.6-bio

    Plasmid
    #68513
    Purpose
    Expresses full-length P. vivax MSP7.6 (PVX_082675) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    MSP7.6
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    68513-plasmid-map-sequence-id-120620

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  45. MSP7.1-bio

    Plasmid
    #68512
    Purpose
    Expresses full-length P. vivax MSP7.1 (PVX_082700) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    MSP7.1
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    68512-plasmid-map-sequence-id-120618

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  46. PP-HSP70 (3L6Q)

    Plasmid
    #25289
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:S12-N394 (cgd2_20 Cryptosporidium parvum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S12-N394
    Promoter
    Available since
    Sept. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. PP-HSP70 (3L4I)

    Plasmid
    #25291
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:G15-E396
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acids G15-E396
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1 - 50 of 715 results