We narrowed to 7,702 results for: 104

1. px330-GAPDH

   Plasmid

   #136940

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting GAPDH (GAPDH Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV-IRES-GFP/CALR del52

   Plasmid

   #214700

   Purpose

   Mammalian expression of human CALR del52

   Depositor

   Norio Komatsu

   Article

   Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

   Insert

   CALR (CALR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-GFP/CALR wt

   Plasmid

   #214699

   Purpose

   Mammalian expression of human CALR wt

   Depositor

   Norio Komatsu

   Article

   Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

   Insert

   CALR wt (CALR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ADD1

   Plasmid

   #155397

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ADD1 (ADD1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-mPCSK3 H194L

   Plasmid

   #122689

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Histidine 194 to Leucine

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-mPCSK3 S368A

   Plasmid

   #122680

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 368 to Alanine

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH127

   Plasmid

   #162483

   Purpose

   SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

   Depositor

   Bob Goldstein

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
   

   Use

   CRISPR

   Tags

   myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQCXIH-Myc-YAP-del C

   Plasmid

   #33095

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deleted C terminal (amino acids 291-450)

   Promoter

   CMV/MSV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW57.1-Cdx2-3xT7

    Plasmid

    #200891

    Purpose

    Inducible lentiviral expression of Cdx2

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0915

    Plasmid

    #143451

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA2 (NCOA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

    Plasmid

    #182231

    Purpose

    Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Inserts

    ras homolog family member B (RHOB Human)
    rhotekin RHO binding domain (RBD)
    

    Use

    Lentiviral

    Tags

    GFP10 (split-GFP tripartite) and GFP11 (M4)

    Expression

    Mutation

    Promoter

    TRE bidirectional

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MC5R-Tango

    Plasmid

    #66431

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC5R (MC5R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pssGFP-hCRT

    Plasmid

    #80022

    Purpose

    Mammalian expression of signal peptide-EGFP-humanCRT

    Depositor

    Chinten James Lim

    Article

    Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

    Insert

    CRT (CALR Human)
    

    Use

    Tags

    EGFP and signal peptide

    Expression

    Mammalian

    Mutation

    signal peptide 17aa added before GFP-CRT, will ta…

    Promoter

    CMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLKO.1 shETV5-B

    Plasmid

    #74978

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR83-Tango

    Plasmid

    #66376

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR83 (GPR83 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. YA1531: pAAV_CAG-FLEX-QuasAr3

    Plasmid

    #107701

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.5 shETV5-A

    Plasmid

    #74977

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD109-bio-His

    Plasmid

    #51862

    Purpose

    Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD109 (CD109 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. QSOX1-bio-His

    Plasmid

    #51825

    Purpose

    Expresses full-length Sulfhydryl oxidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    QSOX1 (QSOX1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBabepuro3-p18 A61D

    Plasmid

    #24937

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A61D

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pMSCV-IRES-hCD2-CD14 (4R)

    Plasmid

    #133062

    Purpose

    Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD14 (4R) (Cd14 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    CD14 allele defective for LPS binding (mutations …

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTD80

    Plasmid

    #172901

    Purpose

    Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YA1629: pAAV_CAG-FLEX-paQuasAr3-s

    Plasmid

    #107703

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF1738

    Plasmid

    #143311

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Human b4 nicotinic receptor subunit EM construct

    Plasmid

    #163961

    Purpose

    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

    Depositor

    Ryan Hibbs

    Article

    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

    Insert

    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    StrepII tag and apocytochrome b(562)RIL (BRIL)

    Expression

    Mammalian

    Mutation

    Pro341-Ser395 replaced with apocytochrome b(562)R…

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAcGFP-Hyg-CTCF-Nterm-N1

    Plasmid

    #131800

    Purpose

    Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 796-2181 (the N-terminus is l…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 3I91

    Plasmid

    #62514

    Purpose

    Bacterial expression plasmid for use in structural determination.

    Depositor

    Cheryl Arrowsmith

    Article

    Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. TFORF2495

    Plasmid

    #142113

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB18 (ZBTB18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO_HOXB13_#2

    Plasmid

    #70094

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pLKO_HOXB13_#1

    Plasmid

    #70093

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ET8-GPIba-6His

    Plasmid

    #102878

    Purpose

    Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus

    Depositor

    Timothy Springer

    Article

    Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1

    Insert

    Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJH130

    Plasmid

    #162484

    Purpose

    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. nes714tk/lacZ

    Plasmid

    #47614

    Purpose

    used to create transgenic mice expressing LacZ reporter with Nestin enhancer

    Depositor

    Urban Lendahl

    Article

    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

    Insert

    nestin 2nd intron fragment (NES Human)
    

    Use

    Enhancer reporter

    Tags

    HSV tk promoter and lacZ

    Expression

    Mutation

    714 bp from 3' end of 2nd intron

    Promoter

    HSV tk promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

37. tetO-ESRRG

    Plasmid

    #170684

    Purpose

    doxycycline-inducible overexpression of ESRRG

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pD649-HAsp-SEMA3C-COMP5AP-AviTag-9xHis

    Plasmid

    #157525

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3C (SEMA3C Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FusionRed-Caveolin-C-10

    Plasmid

    #56103

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. EPHA3_1-972 pcDNA3.1

    Plasmid

    #102739

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge