We narrowed to 12,031 results for: cel.2

1. pCS2 Notch1 LNG (CC→SS)-6MT

   Plasmid

   #41739

   Depositor

   Raphael Kopan

   Article

   Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

   Insert

   Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 leader peptide (aa 1-23) …

   Promoter

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

2. GFP-PODXL FBM*

   Plasmid

   #195220

   Purpose

   Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PODXL (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H477/R479/S481>AAA

   Promoter

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

   Plasmid

   #193678

   Purpose

   Constitutive retroviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Deletion of SET and Zinc finger domains

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCKO2

   Plasmid

   #125518

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUW-TetO-Fos

   Plasmid

   #131593

   Purpose

   doxycycline-inducible expression of mouse c-Fos in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   FBJ osteosarcoma oncogene (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA4/TO/GFP-TTP

   Plasmid

   #141190

   Purpose

   Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   TTP (ZFP36 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HS-GFP-PACT

   Plasmid

   #105954

   Purpose

   heat shock inducible transgenesis, PACT is a centriol marker

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   PACT (AKAP9 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTEF:ATP

   Plasmid

   #92179

   Purpose

   HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
   

   Use

   Integration and expression into yeast.

   Tags

   Yeast codon optimized mseCFP (donor) and cp173-mV…

   Expression

   Yeast

   Mutation

   Promoter

   TEF1p

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sec61β-Flag-APEX2-SOPP3

   Plasmid

   #229072

   Purpose

   Expresse APEX2-SOPP3 in ER in mammalian cell

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the ER (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80062

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HER3-DMVF-181-R

    Plasmid

    #87621

    Purpose

    C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    HER3-DMVF181-C (ERBB3 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCCLc-U6-shHMGA2.3-PGK-dTomato

    Plasmid

    #89606

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

    Plasmid

    #89607

    Purpose

    Expression of LIN28A in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    LIN28A (LIN28A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCLc-U6-shHMGA2.2-PGK-dTomato

    Plasmid

    #89605

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-CARMA2cl-FLAG

    Plasmid

    #97077

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-CARMA2cl-HA

    Plasmid

    #97195

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ECM2-bio-His

    Plasmid

    #53425

    Purpose

    Expresses full-length Extracellular matrix protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ECM2 (ECM2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-PODXL FBM*/PBM*

    Plasmid

    #195221

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dual mutant for HRS>AAA / lacking DTHL

    Promoter

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AdMKP-3 S159AS197A

    Plasmid

    #131523

    Purpose

    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 159 to Alanine and Serine 197 to A…

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiPGK Puro DEST JNKKTRClover

    Plasmid

    #59151

    Purpose

    Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid

    #164903

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y40C mutation in the Rac1 domain whcih make the s…

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMTB2-Avi-Cerulean-RanGAP

    Plasmid

    #79878

    Purpose

    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid

    #79882

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid

    #79881

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid

    #79880

    Purpose

    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. IL2-mCherry-rGBD in pmCherryN1

    Plasmid

    #187286

    Purpose

    Express IL2-Rhotekin GTPase-binding domain-mCherry

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. HA-hNM2B

    Plasmid

    #236533

    Purpose

    For expression of NM2B (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    MYH10 (MYH10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGEM-HE-h_bPAC_cmyc

    Plasmid

    #28134

    Purpose

    optogenetic tool for light modulation of cellular cAMP

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    humanized photoactivated adenylyl cyclase
    

    Use

    In vitro transcription

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHAGE DN Cullin3

    Plasmid

    #41913

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    Cullin 3 (CUL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHAGE DN Cullin4B

    Plasmid

    #41915

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL4B (CUL4B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRS306-pHIS3-2xGFP-tURA3

    Plasmid

    #116936

    Purpose

    Yeast integrative vector for the expression of 2xGFP

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    2xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pT7-HISx6-SIMx6

    Plasmid

    #116938

    Purpose

    Bacterial expression vector for 6 sim

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    6 sim
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pT7-HISx6-SUMOx10

    Plasmid

    #116937

    Purpose

    Bacterial expression vector for 10 sumo

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10 SUMO
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge