9,053 results

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pFR_CrPV_xb

Plasmid

#11509

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

March 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-HsPeg10rc3

Plasmid

#174859

Purpose

Expresses recoded human Peg10 in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

recoded Homo sapiens PEG10 (PEG10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Human, Aequorea victoria)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG:GPI-GFP

Plasmid

#32601

Depositor

Anna-Katerina Hadjantonakis

Article

Rhee et al Genesis. 2006 Apr;44(4):202-18.

Insert

GPI-GFP

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-ER-LAR-GECO1

Plasmid

#61244

Purpose

Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1

Tags

ER-retention sequence: KDEL and ER-targeting sequ…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: V51W/I113V/N35…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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MS2_GFP

Plasmid

#61764

Purpose

Expresses MS2 coat protein fused to GFP

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

MS2 coat protein fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5B

Plasmid

#61802

Purpose

Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

RAB5B (RAB5B Human)

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRASV12

Plasmid

#20205

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

NRas (NRAS Human)

Use

Transposon

Mutation

G12V

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc CIITA (P#808)

Plasmid

#14650

Depositor

Matija Peterlin

Article

Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

Insert

CIITA (CIITA Human)

Tags

Myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(T17N)

Plasmid

#13721

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(T17N) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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Cdc2-DN-HA

Plasmid

#1889

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1b

Plasmid

#1031

Depositor

Bruce Spiegelman

Article

Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

Insert

PGC-1 beta (Ppargc1b Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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