8,812 results

1. pQCXIP-BSR-GFP11

   Plasmid

   #68716

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   bsr
   

   Use

   Retroviral

   Tags

   FLAG and GFP11

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-MET

   Plasmid

   #23889

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MET (MET Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-Lifeact-7

   Plasmid

   #54610

   Purpose

   Localization: Actin, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mEGFP-Lifeact

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMXs-mRFP1

   Plasmid

   #21315

   Purpose

   MoMLV-based Retroviral vector, expresses mRFP1

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   monomeric Red Fluorescence Protein 1
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet30b-5M SrtA

   Plasmid

   #51140

   Purpose

   expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Article

   Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFZ19

   Plasmid

   #36184

   Depositor

   Daniel Voytas

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

   Type

   Empty backbone

   Use

   Plant t-dna vector

   Expression

   Plant

   Promoter

   XVE

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDawn

   Plasmid

   #43796

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. G5-CD40

   Plasmid

   #121166

   Purpose

   pMP71 vector expressing GFP - P2A - G5 - myc - CD40

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   G5-CD40
   

   Use

   Retroviral

   Tags

   GFP and myc

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. myc-hULK1

    Plasmid

    #31961

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    F74G

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-FLEX-GTB

    Plasmid

    #26197

    Depositor

    Edward Callaway

    Article

    Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

    Insert

    Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Mutation

    TVA, rabies glycoprotein are mammalian codon opti…

    Available Since

    Nov. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJC175e

    Plasmid

    #79219

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PPSP gIII, luxAB
    

    Expression

    Bacterial

    Promoter

    PPSP

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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