We narrowed to 5,624 results for: SUP

1. pDONR221_SLC22A1

   Plasmid

   #132034

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

   Plasmid

   #113867

   Purpose

   Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   TOP (wnt dependent promoter)
   NLuc
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEAST-J23101-sfGFP

   Plasmid

   #116917

   Purpose

   Strong constitutive expression of super-folder GFP for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   J23101

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTD-C_sfYFPTwinStrep

   Plasmid

   #45943

   Purpose

   expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Insert

   synthetic sfYFP (codon usage adapted to P.putida KT2440)
   

   Use

   Tags

   Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq/Ptrc

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI pHluorin Syt11

   Plasmid

   #184512

   Purpose

   Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 11 (Syt11 Mouse)
   

   Use

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ires-eGFP(+HAs) donor-1

   Plasmid

   #87865

   Purpose

   Express eGFP reporter for HDR-induced knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Homologous recombination for gapdh loci

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-VHL W117R-pBabe-puro

   Plasmid

   #19239

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tryptophan 117 to Arginin

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 T7 Jip1

   Plasmid

   #51699

   Purpose

   JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP C3-Lats1-PY1+2 mutant

    Plasmid

    #19054

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Large tumor suppressor kinase 1, N mutant (LATS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PY1 mutant, (Tyrosine 376 changed to Alanine) and…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. p2xFlag CMV2 Lats1-PY1+2 mutant

    Plasmid

    #18975

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Large tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    PY1 mutant, (Tyrosine 376 changed to Alanine) and…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-EF1α-DIO-SN

    Plasmid

    #181741

    Purpose

    AAV vector for expressing SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-sfGFP[N150TAA]

    Plasmid

    #164581

    Purpose

    superfolder GFP with N150TAA mutation

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N150TAA Mutation

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Use

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Promoter

    Available since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET28-sfGFP-DogTag Loop B

    Plasmid

    #171780

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSR-puro-Mff1 shRNA

    Plasmid

    #37247

    Depositor

    David Kashatus, Christopher Counter

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Mff1 (MFF Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRJPaph-lacZYA

    Plasmid

    #67037

    Purpose

    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pP19 (GB0038)

    Plasmid

    #68195

    Purpose

    Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLAG GLTP

    Plasmid

    #170740

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRJPaph-gusA

    Plasmid

    #67036

    Purpose

    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-VHL Y98N-pBabe-puro

    Plasmid

    #19236

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Asparagin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-Hook3 aa 1-160-GCN4

    Plasmid

    #74608

    Purpose

    Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, GCN4, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-VHL Y112N-pBabe-puro

    Plasmid

    #19238

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Asparagin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-VHL Y112H-pBabe-puro

    Plasmid

    #19237

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Histidin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1P shABCB7_2

    Plasmid

    #102969

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_2 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1P shABCB7_1

    Plasmid

    #102968

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_1 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BAS292 C terminal SF-GFP kanR

    Plasmid

    #74087

    Purpose

    S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    SF-GFP recoded for S.pombe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

32. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. spa-GCaMP6s

    Plasmid

    #67556

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pRJPaph-mTq2

    Plasmid

    #67031

    Purpose

    Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mTurqoise2
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEN-R2-mCsfGFP-L3

    Plasmid

    #178602

    Purpose

    It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

    Depositor

    Frederica Theodoulou

    Article

    Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

    Insert

    Tandem fluorescent protein timers (tFTs)
    

    Use

    Entry vector for gateway cloning

    Tags

    mCherry superfolder GFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ninaE-dsocs

    Plasmid

    #26857

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    Slow Termination of Phototransduction (stops Fly)
    

    Use

    Tags

    myc

    Expression

    Insect

    Mutation

    This STOPS construct contains a mutated/truncated…

    Promoter

    Available since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

37. HA-VHL L188V-pBabe-puro

    Plasmid

    #19241

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Leucin 188 to Valin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-VHL Y98H-pBabe-puro

    Plasmid

    #19235

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Histidin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSK260

    Plasmid

    #129252

    Purpose

    dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35 NM yTRAP sensor reporting on mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-VHL 1-115-pBabe-puro

    Plasmid

    #19243

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 116 - 213 (end)

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

42. pSR-siKlf5

    Plasmid

    #41740

    Depositor

    Jonathan Katz

    Article

    Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

43. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid

    #107421

    Purpose

    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    mutation of glutamate 77 to glutamine

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSK247

    Plasmid

    #131148

    Purpose

    dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSUP35, min pCYC1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28-sfGFP-SpyTag003 Loop A

    Plasmid

    #171776

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues V…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. sspa-GCaMP6f

    Plasmid

    #67561

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

48. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pRJPaph-HcRed

    Plasmid

    #67035

    Purpose

    Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    HcRed
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSR353

    Plasmid

    #125078

    Purpose

    Expression of narL under PLtetO-1, output promoter PdcuS77

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL (narL )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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