We narrowed to 30,432 results for: Ide

1. mCherry-CRY2-INPP5E

   Plasmid

   #79561

   Purpose

   expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

2. mCherry-CRY2-INPP5E(D556A)

   Plasmid

   #79563

   Purpose

   expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCJ217

   Plasmid

   #162685

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   G489C, S530C; codon optimized for E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-mFzd8

   Plasmid

   #102870

   Purpose

   Expresses mouse Fzd8 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Frizzled 8 (Fzd8 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T to C substitution at nucleotide 1134, C to T su…

   Promoter

   EF1A

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ200

   Plasmid

   #162673

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C and a sequence Y75-F104 to introduce a 6th…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ210

   Plasmid

   #162683

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C, G489C, S530C, and a sequence Y75-F104 to i…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK034

   Plasmid

   #72383

   Purpose

   Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Insert

   phosphoribosylglycinamide synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-RupA_T1-NHis

   Plasmid

   #109259

   Purpose

   NRPS T domain with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Schneider et al Tetrahedron

   Insert

   RupA_T1
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   contains aa 917..1025 of WP_015523502.1

   Promoter

   T7

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-RupA_R-NHis

   Plasmid

   #109257

   Purpose

   NRPS R domain with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Schneider et al Tetrahedron

   Insert

   RupA_R
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   contains aa 2038..2443 of WP_015523502.1

   Promoter

   T7

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJQ210SK

    Plasmid

    #78499

    Purpose

    Cosmid version of suicide vector pJQ200SK. Suicide vector

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector

    Tags

    none

    Promoter

    non

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET29b-RupA_T2-R-CHis

    Plasmid

    #109258

    Purpose

    NRPS di-domain containing TR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T2-R
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1939..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-RupA_C2-A2-T2-R-CHis

    Plasmid

    #109256

    Purpose

    NRPS module containing CATR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C2-A2-T2-R
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1023..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29b-RupA_C1-A1-T1-CHis

    Plasmid

    #109255

    Purpose

    NRPS module containing CAT domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C1-A1-T1
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1..1025 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKB225

    Plasmid

    #170015

    Purpose

    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Peptide display protein with no C-terminal peptide
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. iRFP-PH-PLCdelta1

    Plasmid

    #66841

    Purpose

    expression of near-infrared PI(4,5)P2 biosensor

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    PH-PLCdelta1
    

    Tags

    iRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYAL_HLA-A2_MART1

    Plasmid

    #171177

    Purpose

    Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

    Depositor

    Michael Birnbaum

    Article

    Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

    Insert

    MART1_HLA-A2*01 single-chain trimer fusion
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-SwiChRca-TS-EYFP

    Plasmid

    #55630

    Purpose

    Chloride Channel

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

    Insert

    iC1C2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128A

    Promoter

    CaMKIIa

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYHY2

    Plasmid

    #216108

    Purpose

    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT0294 signal peptide

    Expression

    Bacterial

    Promoter

    BfP1E6 promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLacI

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pPGK1

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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