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163,584 results

Showing: 2081 - 2100 of 163584 results

  3. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid
    #115684
    Purpose
    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.
    Depositor
    David Suter
    Article
    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1
    Insert
    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115684-plasmid-map-sequence-id-224735

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  5. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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  9. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Article
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103056-plasmid-map-sequence-id-199055

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  12. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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  14. pM2s2TsR

    Plasmid
    #137923
    Purpose
    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    137923-plasmid-map-sequence-id-322897

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  15. pM1s3AsG

    Plasmid
    #137921
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    137921-plasmid-map-sequence-id-322876

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  16. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  17. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available Since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

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  18. TFORF3195

    Plasmid
    #144671
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT1 (ONECUT1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144671-plasmid-map-sequence-id-350517

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Showing: 2081 - 2100 of 163584 results