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Active Wnt5B-V5

Plasmid

#43814

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT5B (WNT5B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD42-BtuCEDFB

Plasmid

#237541

Purpose

plasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteins

Depositor

Squire Booker

Article

Lanz et al Biochemistry. 2018 Mar 6;57(9):1475-1490

Inserts

btuB
btuF
btuCED

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAJM.657

Plasmid

#108525

Purpose

CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CymRAM + PCymRC-YFP

Expression

Bacterial

Mutation

CymR: S110G A171V

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

AAVS1-specific sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_D210N

Plasmid

#111904

Purpose

Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Article

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Available Since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS-rtTA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NanoLuc-BRD9 Fusion Vector

Plasmid

#238874

Purpose

Express NanoLuc-BRD9 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

BRD9 (BRD9 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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FLX1.8NFATGFPpd2HS4

Plasmid

#169096

Purpose

Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

Depositor

Geoffrey Owens

Inserts

NFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein

Use

Lentiviral

Promoter

NFAT enhancer + synthetic TATA box

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-WT

Plasmid

#111575

Purpose

Expresses full length PARP1 in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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cytoplasmic cpVenus control

Plasmid

#186788

Purpose

Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

cytoplasmic cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ATLASsnCre

Plasmid

#232351

Purpose

Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Has Service

AAV5

Insert

ATLASsnCre

Use

AAV

Tags

ALFA-Tag

Promoter

hSyn

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_FUS

Plasmid

#26374

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only