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pCAGGS-FLAG-mISG15

Plasmid

#12443

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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5` CC: Puro – loxP – GFP-C

Plasmid

#219563

Purpose

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

hPGK-promoter_PuroR_2A_loxP_GFP-C

Use

Unspecified

Promoter

hPGK

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rtn4b-GFP

Plasmid

#186952

Purpose

Expresses Rtn4b connected with C-terminus AcGFP1

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

RTN4B (RTN4 Human)

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHIVT9-NAT-3xFlag-SMARCAL1

Plasmid

#246935

Purpose

Lentiviral vector that expresses wildtype Flag-tagged SMARCAL1

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPM_Pveg-sfgfp

Plasmid

#121503

Purpose

Integration vector that expresses gfp in S. mutans

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

Superfolder GFP

Use

S. mutans integration vector, integrates at the m…

Promoter

Pveg

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV INS2x mRFP-3MREs (AAV-beta)

Plasmid

#206928

Purpose

Beta cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Article

Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

INSx2

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-C1

Plasmid

#54842

Purpose

Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Tags

mTurquoise2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hsyn-GRAB_HA1h

Plasmid

#190473

Purpose

Express the high-affinity histamine sensor GRAB_HA1h in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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6xHsa.NFKB:d2mRFP1

Plasmid

#239991

Purpose

Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

Depositor

Raquel Espin-Palazon

Article

Campbell et al Nat Commun. 2024 Sep 6;15(1):7787. doi:

Inserts

mRFP1
PEST

Promoter

cfos minimal promoter

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_3RZY

Plasmid

#234156

Purpose

Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli

Depositor

Christopher Bahl

Insert

3RZY (FABP4 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro

Plasmid

#204731

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/IL-2/Hygro] Vector

Plasmid

#236930

Purpose

Express IL-2 in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

IL-2 (IL2 Human)

Use

Luciferase

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PIP5K1C (PIP5K1C Human)

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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