We narrowed to 16,885 results for: Por

1. Plasma membrane-targeted optodPLD

   Plasmid

   #140061

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Promoter

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5_tAKARβ

   Plasmid

   #119914

   Purpose

   Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARβ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET sfGFP(150TAG)

   Plasmid

   #133455

   Purpose

   expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

   Depositor

   Yu-Hsuan Tsai

   Article

   Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

   Insert

   sfGFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFH2.30_Gag-MCP-dpol-T2A-eGFP

   Plasmid

   #205538

   Purpose

   Express HIV Gag-MCP-dpol-T2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   HIV Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pol frameshift sequence recoded

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM552

   Plasmid

   #154131

   Purpose

   Expresses wt rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   wt bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKanCMV-mClover3-10aa-H2B

   Plasmid

   #74257

   Purpose

   Mammalian expression of N-terminal fusion of mClover3 to H2B

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   H2B
   

   Use

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Use

   Tags

   iRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

   Plasmid

   #214989

   Purpose

   Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFP L202 gRNA

   Plasmid

   #119132

   Purpose

   gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   eGFP L202 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   U6

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJAM1.18_PB-SDC4-PGK-Blast

    Plasmid

    #205563

    Purpose

    Express human SDC4

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107131

    Purpose

    cGAL-N driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFPZFN1.4-B2H

    Plasmid

    #26791

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202023

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-I48V

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFPZFN2-B2H

    Plasmid

    #26792

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. CMV-PKCb1-mEGFP-N1

    Plasmid

    #112265

    Purpose

    ITRACKbeta/IDOCKSbeta Donor construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c beta (Prkcb Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD/HisB-Fast-FT

    Plasmid

    #31916

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    araB

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. (R42A) Cyclin B1-GFP (62)

    Plasmid

    #61849

    Purpose

    mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

    Depositor

    Jonathon Pines

    Article

    Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    V5A R42A

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107130

    Purpose

    cGAL-C driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWXLd-RIEP-CeNLuc

    Plasmid

    #135934

    Purpose

    Expresses the CeNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

    Plasmid

    #205760

    Purpose

    Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    YFP-SLBP(18-126)-IRES-H1-Skylan62A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAC-GGPPipi

    Plasmid

    #53280

    Purpose

    Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107132

    Purpose

    cGAL-N driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107136

    Purpose

    cGAL-C driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKin1B

    Plasmid

    #31604

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    kinesin-1B (Kif5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205552

    Purpose

    Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP-p6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-ICUE3-NLS

    Plasmid

    #138218

    Purpose

    Nuclear-targeted FRET-based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    ICUE3-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

    Plasmid

    #213119

    Purpose

    Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJAK112

    Plasmid

    #167280

    Purpose

    CodA-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKHR5

    Plasmid

    #74593

    Purpose

    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-crystallin

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-2H12.D11

    Plasmid

    #221145

    Purpose

    His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. P305-FBP_4

    Plasmid

    #139695

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Mut2-RS

    Plasmid

    #182882

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut2-RS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDAS12124_PEAR-GFP-preedited

    Plasmid

    #177179

    Purpose

    PEAR-GFP control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pPBCAG-rtTAM2-IH

    Plasmid

    #140827

    Purpose

    Expresses rtTAM2-IRES-HygR in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    rtTAM2
    HygR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCCl-MCS_miniCMV_TagBFP

    Plasmid

    #134986

    Purpose

    The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pPIDCB2

    Plasmid

    #169900

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available Since

    Dec. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLD-puro-CcVA

    Plasmid

    #24588

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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48. p2H12

    Plasmid

    #221142

    Purpose

    cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEN_TT 3xFLAG-XRCC6

    Plasmid

    #192302

    Purpose

    Gateway entry vector for an inducible 3xFLAG-XRCC6

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    XRCC6 (XRCC6 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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