We narrowed to 52,823 results for: plasmid
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Plasmid#18458DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationR96MPromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme R96S
Plasmid#18461DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationR96SPromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme A129V
Plasmid#18573DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationA129VPromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L39P
Plasmid#18292DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationL39PPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme K60P
Plasmid#18368DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationK60PPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme I3D
Plasmid#18204DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationI3DPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme K16E
Plasmid#18230DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationK16EPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme P37A
Plasmid#18282DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationP37APromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRD183 (Roc2)
Plasmid#14824DepositorInsertRoc2 (Roc2 Fly)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2
Plasmid#231904PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERbeta-5Z
Plasmid#23062DepositorInsertERbeta (ESR2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB235
Plasmid#164130PurposePhlF-RFP expression plasmidDepositorInsertPhlF-RFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_NSP16-VA
Plasmid#191215PurposeLentiviral expression of SARS-CoV-2 NSP16 in mammalian cellsDepositorInsertNon structural protein 16 (ORF1ab SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
CFTR-HiBiT Fusion Vector
Plasmid#236890PurposeExpress CFTR WT HiBiT in Mammalian Cells under a CMV promoterDepositorUseTagsHiBiTExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, TREM2-HaloTag
Plasmid#237148PurposeExpress TREM2-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p-dCas9-HDAC1-H141A-Hygro
Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorInsertdHDAC1 (HDAC1 Human)
UseCRISPRTagsExpressionMammalianMutationH141APromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT10
Plasmid#204293PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO
Plasmid#199781PurposePlasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).DepositorInsertNEMO (IKBKG Human)
UseTagsGST-Thrombin-TEV-EGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.wt
Plasmid#110346PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP-HuR.wt
Plasmid#110349PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-Hygro
Plasmid#104409Purposetransient expression of dCas9-HDAC1 fusion proteinDepositorInsertHDAC1 (HDAC1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
Plasmid#85225PurposeExpresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsUseAAV and Cre/LoxTagsExpressionMutationPromoterhSynAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
NanoBRET Assay Vector, HaloTag-APC
Plasmid#237145PurposeExpress HaloTag(R)-APC Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
KSR1-HaloTag Fusion Vector
Plasmid#236794PurposeExpress KSR1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLZ12Km2-P23R:TA:Xen5
Plasmid#88902PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + LuxABCDE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SJZ5-HPH
Plasmid#228258PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin BDepositorInserthph
UseTagsExpressionYeastMutationPromoterSSA1 S.c.Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Bla-Ak-ACE2-TMPRSS2
Plasmid#154983PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT39
Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA2-scFv-mNeon-KDEL
Plasmid#230057PurposeAAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SJZ9-amdS
Plasmid#228261PurposeTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen sourceDepositorInsertamdS
UseTagsExpressionYeastMutationPromoterENO2 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJ1021
Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cas
Plasmid#50729Purposemammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCas (Bcar1 Rat)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA1-scFv-mNeon-KDEL
Plasmid#230056PurposeAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSP-GM1
Plasmid#64739PurposeYeast expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverexpression of Flp step-arrest mutant in yeast, URA selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
6xO/S luc
Plasmid#69445Purposedetection/assessment of OCT4 and SOX2 transcription factor activityDepositorInsertminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only