We narrowed to 10,942 results for: NSI;

1. Blue opsin mcherry ---- pcDNA3.1

   Plasmid

   #62932

   Purpose

   Blue opsin tagged with mcherry on the C terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   blue opsin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMS2CP(V29I)-myc-T2A-tagRFP

   Plasmid

   #140260

   Purpose

   Expresses MS2CP(V29I) in mammalian cells

   Depositor

   Hirohide Saito

   Article

   Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

   Insert

   Coat protein of bacteriphage MS2
   

   Use

   Synthetic Biology

   Tags

   myc-T2A-tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PAmCherry1-N1-PHPLCd1

   Plasmid

   #202679

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5-mFzd9

   Plasmid

   #42261

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 9 (Fzd9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

5. CrBlue ---- pcDNA3.1

   Plasmid

   #62917

   Purpose

   CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   CrBlue
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMC-1-EXOB-11

   Plasmid

   #197688

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EXOB enhancer-blocking insulator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EK0501 SFFV-mCherry-SGc(MYC.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191185

   Purpose

   Expression of MYC sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:MYC.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

   Plasmid

   #140221

   Purpose

   Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1 (Cas12a) N terminal half (406) with zipper
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB045a

   Plasmid

   #79224

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ rpoZ-HA4; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ; PlacIq

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAB094e

    Plasmid

    #79245

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4 (Y87A); PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRXG

    Plasmid

    #113643

    Purpose

    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHS-393

    Plasmid

    #195473

    Purpose

    Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUMO-LIC-CHIKV-nsP2h

    Plasmid

    #127935

    Purpose

    Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

    Depositor

    Dahai Luo

    Article

    Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

    Insert

    CHIKV nsP2h
    

    Use

    Tags

    His6-Sumo-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-mFzd6

    Plasmid

    #42258

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 6 (Fzd6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. GLUT9-eGFP/pcDNA-DEST47

    Plasmid

    #18730

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pRK5-mFzd4

    Plasmid

    #42256

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAID1.2-CMV-CGFP-mAID

    Plasmid

    #140606

    Purpose

    Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. raACR-pmCerulean3-N1

    Plasmid

    #204959

    Purpose

    Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TRE-DIO-proCaspase-3-TEVp

    Plasmid

    #166607

    Purpose

    Encodes Cre-dependent procaspase 3 and TEVp under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    procaspase3- TEVp
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK-2-Zeb2-3'UTR

    Plasmid

    #61771

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zeb2

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zeb2 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pAID1.2-CMV-CmCherry-mAID

    Plasmid

    #140602

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 3xEGFP-mMeikin

    Plasmid

    #174708

    Purpose

    Mammalian expression of mouse Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Use

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAB035h

    Plasmid

    #79191

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 gIII, luxAB; Ptet rpoZ-HA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ptet

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBFP (GB0025)

    Plasmid

    #68193

    Purpose

    Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    BFP coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-TRE-DIO-TVA-oG-mCherry

    Plasmid

    #166602

    Purpose

    Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    TVA-oG-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p426 46Q GPD

    Plasmid

    #1182

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 46Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pSB1K3_pBen-sfGFP_J23101-mRFP

    Plasmid

    #128126

    Purpose

    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBen (benzoate responsive promoter)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-TRE-DIO-eGFP-L10a

    Plasmid

    #166605

    Purpose

    Encodes Cre-dependent eGFP-L10a under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    eGFP-L10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWJB30

    Plasmid

    #126505

    Purpose

    Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD30

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJW4.02

    Plasmid

    #131372

    Purpose

    expression of MBP:PGL-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pgl-1 (pgl-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)-sapphireAKAR

    Plasmid

    #118464

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO mouse ULK2 shRNA 93

    Plasmid

    #27635

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK2 shRNA 93
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQE80l A-CC-A

    Plasmid

    #196478

    Purpose

    Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    A-CC-A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-TRE-DIO-GFP-2A-SynmRuby

    Plasmid

    #166608

    Purpose

    Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    GFP-2A-syn
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1P shTFRC

    Plasmid

    #169882

    Purpose

    Suppress TRFC

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against TFRC
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB9336 (pgRNA_II-1_NatMX)

    Plasmid

    #161589

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge