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Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1
Plasmid#27156PurposeFor mammalian expression of human Elk-1 with an HA tagDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHSE401
Plasmid#62201PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (m) Vector
Plasmid#101135PurposeExpression of protein of interest with SNAP-Tag in Mammalian cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26m)ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 hygro
Plasmid#24150Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19
Plasmid#50005PurposepUC cloning vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-PEmax for IVT
Plasmid#178113PurposeTemplate for in vitro transcription of PEmaxDepositorInsertPEmax
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpo
Plasmid#60662PurposeExpresses pCAG-FlpoDepositorInsertFlpo
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDA_174
Plasmid#136476PurposeLentiviral expression of EnAsCas12aDepositorInsertEnAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianMutationPromoterU6 and hSynAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only