4,203 results

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pET-45b(+)-Tn5

Plasmid

#112112

Purpose

Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

Depositor

Frank Pugh

Article

Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

Insert

Tn5

Tags

His6

Expression

Bacterial

Mutation

E54K E110K P242A L372P

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3A

Plasmid

#35521

Purpose

Mammalian expression of human DNMT3A with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3A (DNMT3A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B1

Plasmid

#35522

Purpose

Mammalian expression of human DNMT3B1 with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B1 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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3XMEF2-luc

Plasmid

#32967

Purpose

Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

Depositor

Ron Prywes

Insert

MEF2 binding sites (MEF2A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

MEF2

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-Vimentin-7

Plasmid

#56439

Purpose

Localization: Intermediate Filaments, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-myc-his-BACH1 WT

Plasmid

#17642

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pPGKPhiC31obpA

Plasmid

#13795

Purpose

Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

PhiC31o

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3/UBC9

Plasmid

#20082

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

Insert

UBC9 (UBE2I Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R273H

Plasmid

#16439

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R273H (TP53 Human)

Expression

Mammalian

Mutation

R273H

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Expression

Mammalian

Mutation

R248W

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-BP1-2 pLPC-Puro

Plasmid

#19835

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

Fragment of p53-Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

mCherry

Mutation

Fragment of human 53BP1 aa 1220 - 1711

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Article

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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AIO-Puro

Plasmid

#74630

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Puromycin resistant marker

Expression

Mammalian

Promoter

Cbh

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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HIR WT

Plasmid

#24049

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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