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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-Tre3G-29xGGGGCC-12xMS2

Plasmid

#99149

Purpose

Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

29xGGGGCC 12xMS2 hairpins

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Use

Tags

Expression

Plant

Mutation

Promoter

35S CaMV

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1

Plasmid

#36412

Depositor

Benjamin Glick

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBMM42

Plasmid

#13331

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

XRCC4 (XRCC4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pAS_4xMma PylT_FLAG-Mma PylRS AF

Plasmid

#140023

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y271A, Y349F

Promoter

EF1

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_TFAM_mKate2

Plasmid

#167331

Purpose

Expresses mKate2 fused to full length TFAM in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLZts

Plasmid

#128799

Purpose

Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

Depositor

Timothy Barnett

Article

Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

Type

Empty backbone

Use

Bacterial mutagenesis

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBA904

Plasmid

#122238

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

Depositor

Jonathan Weissman

Article

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs1 in stem loop 2 of the constant region

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLSB-HYG

Plasmid

#166699

Purpose

Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

Depositor

Robin Allshire

Article

Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

Inserts

tRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe

Use

Tags

Expression

Yeast

Mutation

Promoter

adh15 promoter and tRNA promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV14-3X-Flag-SARS-CoV-2 S

Plasmid

#145780

Purpose

Over expression of SARS-CoV-2 S (spike) in mammalian cell line

Depositor

Zhaohui Qian

Article

Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

Insert

SARS-CoV-2 S (S SARS CoV 2)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Codon optimized and C-terminal 19 amino acids del…

Promoter

CMV

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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