167,150 results
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Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
EB3-GFP
Plasmid#190164PurposeExpresses EB3-GFP in mammalian cellsDepositorInsertEB3-GFP
TagsGFPExpressionMammalianAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-N1
Plasmid#54737PurposeLocalization: N1 Cloning Vector, Excitation: 485, Emission: 507DepositorTypeEmpty backboneTagssfGFPExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiExpressionMammalianPromoterU6 promoterAvailable SinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGFPGUSPlus
Plasmid#64401PurposeBinary plant vector expressing GFP and GUS, both driven by a 35S promoterDepositorInsertsGFP
GUSPlus
UseBinary vector for plant transformationTagsHisExpressionPlantMutationS65TPromoter35SAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI
Plasmid#201684PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1 (V106W)
UseAAV and CRISPRExpressionMammalianPromoterU1aAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux1
Plasmid#14083DepositorInsertBacterial luciferase
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3Luc-5XNF-kappaB
Plasmid#185695PurposeReporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').DepositorAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycPromoterGAL1Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2_ubi:EGFP
Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMK435
Plasmid#236573PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_BDepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4-UPRE-luc2P-Hygro
Plasmid#101788PurposeLuciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)DepositorInsert3xUPRE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N1
Plasmid#125547PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to n-terminus of Gatew…ExpressionBacterial and MammalianPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only