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169,365 results

Showing: 2181 - 2200 of 169365 results

  4. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178885-plasmid-map-sequence-id-354971

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  9. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  15. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  16. ER-M-TAG (pGS555)

    Plasmid
    #160040
    Purpose
    pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    MMGT1-mScarlet-3xHA
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160040-plasmid-map-sequence-id-323176

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  19. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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Showing: 2181 - 2200 of 169365 results