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pJFRC2-10XUAS-IVS-mCD8::GFP

Plasmid

#26214

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

Plasmid

#203838

Purpose

Flp-dependent Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mScarlet-KASH-2A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Smad5

Plasmid

#11744

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

SMAD5 (Smad5 Mouse)

Expression

Mammalian

Available Since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV mouse sidekick2

Plasmid

#18735

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Sidekick-2 (Sdk2 Mouse)

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag PGC1alpha S570A

Plasmid

#15988

Depositor

Morris Birnbaum

Article

Li et al Nature. 2007 Jun 21. 447(7147):1012-6.

Insert

PGC1 alpha (PPARGC1A Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S570A

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-1_enzymatic-domain

Plasmid

#183385

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404) (Casp1 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

Plasmid

#192828

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TRE-STOP EF1a-rtTA3

Plasmid

#195882

Purpose

control construct of TRE-VEGFA EF1a-rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-mtIF3 shRNA_TagRFP657

Plasmid

#182367

Purpose

shRNA targeting mtIF3 mRNA

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6 promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHLuc (Antares-SEPLuc C1A4E+6)

Plasmid

#141089

Purpose

final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (F133V)

Plasmid

#169725

Purpose

Mammalian Expression of mouse SPOP F133V

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

F133V

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

Plasmid

#182888

Purpose

Mammalian expression of PVALB mouse mAb HC gene

Depositor

Heng Zhu

Insert

anti-PVALB (human) heavy chain (PVALB Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

No

Promoter

CMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1ADA(pCMV5)

Plasmid

#12149

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

Threonine 24 to Alanine (A) and Serine 253 to Asp…

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only
Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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