19,186 results

1. pMH-HA

   Plasmid

   #101767

   Purpose

   Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. scFLARE1

   Plasmid

   #158738

   Purpose

   Mammalian expression of scFLARE1 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE1
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAX FLAG APP

   Plasmid

   #30154

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wild type splice variant 751

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF1389

   Plasmid

   #104962

   Purpose

   Bacterial expression plasmid of bdSENP1 protease

   Depositor

   Dirk Görlich

   Article

   Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

   Insert

   SENP1
   

   Use

   Tags

   His14 tag and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-FLEx(FRT)-G

   Plasmid

   #67828

   Purpose

   Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   rabies glycoprotein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP GR

   Plasmid

   #47504

   Purpose

   Expresses glucocorticoid receptor in mammalian cells

   Depositor

   Alice Wong

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-N-3XFLAG-Ctnnb1

    Plasmid

    #123586

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT120a17

    Plasmid

    #138510

    Purpose

    P1

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    gIII-xLuxAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSII-EF/mt-(n1)StayGold

    Plasmid

    #185823

    Purpose

    A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    mt-(n1)StayGold
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cx43-msfGFP

    Plasmid

    #69007

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Gja1 (Gja1 Rat)
    

    Use

    Tags

    monomerized superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSP64T (DM#111)

    Plasmid

    #15030

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-pBAD

    Plasmid

    #54859

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pcDNA3.1-ccdB-3xFLAG-V5

    Plasmid

    #87063

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-hHSF1 WT

    Plasmid

    #71724

    Purpose

    plasmid expressing myc-his tag hHSF1 WT

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    MYC 6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMD.RVG.CVS24-B2c

    Plasmid

    #19713

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-B2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. FRE5Cf

    Plasmid

    #62378

    Purpose

    Derived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKD3

    Plasmid

    #45604

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-cat-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R6K

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCIBN(deltaNLS)-pmGFP

    Plasmid

    #26867

    Purpose

    Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-EGFP

    Plasmid

    #89684

    Purpose

    pCAG promoter driving EGFP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-ALK5 wt

    Plasmid

    #80876

    Purpose

    mammalian expression of ALK5 WT

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPROBE-gfp[tagless]

    Plasmid

    #40164

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotor probe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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