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Showing: 4441 - 4480 of 158806 results

  6. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  12. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha and EF1-alpha
    Available since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

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  15. AbVec2.0-mIghg2c

    Plasmid
    #127159
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg2c (Ighg2c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127159-plasmid-map-sequence-id-256903

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  18. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  24. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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  26. TFORF3228

    Plasmid
    #144704
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAX (MAX Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144704-plasmid-map-sequence-id-404307

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  27. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

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  28. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  29. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

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  30. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  34. HAT1

    Plasmid
    #25239
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HAT1_05 (HAT1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4441 - 4480 of 158806 results