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167,150 results

Showing: 4441 - 4480 of 167150 results

  4. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Expression
    Bacterial
    Promoter
    AmpR and lpp
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  5. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  9. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  12. mCherry-Gamma-Tubulin-17

    Plasmid
    #55050
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Gamma-Tubulin (TUBG1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55050-plasmid-map-sequence-id-215600

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  13. Sc RFC[hk-1s + 5]-pLANT/RIL

    Plasmid
    #239200
    Purpose
    Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC1 (RFC1 Budding Yeast)
    RFC5 (RFC5 Budding Yeast)
    Tags
    His-10 + PKA kinase motif on truncated Sc RFC1
    Expression
    Bacterial
    Mutation
    N-terminal truncation
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239200-plasmid-map-sequence-id-476583

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  14. Sc RFC[2+3+4]-pET

    Plasmid
    #239203
    Purpose
    Overexpress Sc RFC2, RFC3, & RFC4 in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC2 (RFC2 Budding Yeast)
    RFC3 (RFC3 Budding Yeast)
    RFC4 (RFC4 Budding Yeast)
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239203-plasmid-map-sequence-id-474660

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  15. pET28b-SUMO-SeKaiA

    Plasmid
    #229218
    Purpose
    For expression of S. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA S. elongatus)
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229218-plasmid-map-sequence-id-457045

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  17. NET29B.mm pRRLSIN (1744)

    Plasmid
    #62053
    Purpose
    lentiviral expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29B (Tmem120b Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62053-plasmid-map-sequence-id-101297

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  18. p4xSTAT6-Luc2P

    Plasmid
    #35554
    Depositor
    Axel Nohturfft
    Insert
    4xStat6 promoter
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    4xStat6
    Available Since
    March 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    35554-plasmid-map-sequence-id-338744

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  23. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid
    #72485
    Purpose
    Express Luc2 and copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72485-plasmid-map-sequence-id-132335

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  24. ADORA2A-Tango

    Plasmid
    #66210
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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Showing: 4441 - 4480 of 167150 results