We narrowed to 6,015 results for: pCas

Showing: 2241 - 2260 of 6015 results

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEN716 - pCAGGS-eGFP-3Xnls-bpA

Plasmid

#156435

Purpose

For transient expression of nuclear eGFP in mammalian cells

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

eGFP

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-Lbr-V5-mCherry

Plasmid

#235094

Purpose

Lbr mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
p2T-cmv-SpCas9-TadCBEa-BlastR

Plasmid

#193847

Purpose

Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-fwd[Chrimson-tdT]

Plasmid

#59137

Purpose

Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-tdTomato

Use

Cre/Lox

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mRuby3-WPRE

Plasmid

#99279

Purpose

Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

Depositor

Rylan Larsen

Insert

mRuby3

Use

AAV and Cre/Lox

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP/RFP-miR-Scrint

Plasmid

#19821

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

Scrambled miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-ST2-com-vector

Plasmid

#136269

Purpose

Expresses SpCas9 and sgRNA Scaffold

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Insert

SpCas9 and sgRNA scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mIRFP670nuc-TEV-Xph20-ETWV

Plasmid

#133044

Purpose

Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

NLS, TEV protease + TEV cleavage site, and miRFP6…

Expression

Mammalian

Mutation

S63K

Promoter

CAG

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-FLCN

Plasmid

#164981

Purpose

FLCN expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PDGFRb559-562del-IRES2-EGFP

Plasmid

#204355

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion).

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Mutation

559-562 deletion

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Expression

Mammalian

Promoter

Human U6

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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