159,238 results

1. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Mustard Weed, Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-APP-mGFP

   Plasmid

   #196696

   Purpose

   Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   HA and mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRH030

   Plasmid

   #159633

   Purpose

   CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

   Depositor

   Robert Hertel

   Article

   Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

   Type

   Empty backbone

   Use

   CRISPR; Shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Constitutive tracrRNA

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPc3 K-RAS 4B

   Plasmid

   #236452

   Purpose

   transient overexpression of K-RAS 4B in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

   Insert

   K-RAS 4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_SETDB1_WT

   Plasmid

   #82291

   Purpose

   Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-NLS-HA-Bxb1

   Plasmid

   #51271

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-U6-sgRNA-EGFP

   Plasmid

   #107721

   Purpose

   For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

    Plasmid

    #192617

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RiboL1-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-HIF1alpha-wt-pBabe-puro

    Plasmid

    #19365

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt human cDNA

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. CL20_mEGFP_RUNX1_RUNX1T1

    Plasmid

    #205915

    Purpose

    Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1444

    Plasmid

    #143809

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARID4B (ARID4B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamp1-mNeonGreen

    Plasmid

    #98882

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pet28a_Human_H3.3

    Plasmid

    #42632

    Depositor

    Joe Landry

    Insert

    Human H3.3 (H3-3A Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHR_AXP_hrGFP

    Plasmid

    #84613

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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