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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Publication

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pSYFP2-C1

Plasmid

#22878

Depositor

Dorus Gadella

Publication

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2

Expression

Mammalian

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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TetO-FUW-Klf4

Plasmid

#20322

Depositor

Rudolf Jaenisch

Publication

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pB18cmBFPAzurite

Plasmid

#14034

Depositor

Patrick Daugherty

Publication

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Insert

Azurite

Use

Yeast/bacteria optimized codons

Tags

6xHis

Expression

Bacterial

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p181 pK7-HDAC1 (GFP)

Plasmid

#11054

Depositor

Ramesh Shivdasani

Publication

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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siSlug3

Plasmid

#10905

Depositor

Bob Weinberg

Publication

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

siSlug (SNAI2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Publication

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Publication

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Expression

Mammalian

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

Plasmid

#84469

Purpose

pAAV-RAM-EGFP

Depositor

Yingxi Lin

Publication

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

EGFP

Use

AAV

Promoter

TRE

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

Plasmid

#84474

Purpose

pAAV-RAM-NLS-mKate2

Depositor

Yingxi Lin

Publication

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

mKate2

Use

AAV

Tags

Nuclear localization signal of SV40 large T antig…

Promoter

TRE

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Publication

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLPCX mito roGFP2-Orp1

Plasmid

#64992

Purpose

Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Publication

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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mIFP-N1

Plasmid

#54620

Purpose

Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Tags

mIFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Publication

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-C1-hAtg2A

Plasmid

#36456

Depositor

Noboru Mizushima

Publication

Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

Insert

autophagy-related protein 2 homolog A (ATG2A Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed Proline 656 to Arginine

Promoter

CMV

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti CMV/TO RasV12 Puro (w119-1)

Plasmid

#22262

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Flag-HA-CYLD

Plasmid

#22544

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Publication

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Publication

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Expression

Mammalian

Available since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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