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pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mouse Oct4

Plasmid

#21110

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Oct4 (Pou5f1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-2T Sam68

Plasmid

#17687

Depositor

David Shalloway

Article

Resnick et al Oncogene. 1997 Sep;15(11):1247-53.

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pDUP 4-1

Plasmid

#23022

Depositor

Douglas Black

Article

Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

Insert

c-src (Src Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1

Plasmid

#61086

Purpose

Express N-terminal GST tagged-mouse BIN1 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

smallest constitutive BIN1excluding exon 7, 11, 13-17

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM3-N-22

Plasmid

#54166

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Anti-Kv1.1 K+ channel [K20/78R]

Plasmid

#128616

Purpose

Mammalian Expression Plasmid of anti-Kv1.1 K+ channel (Rat). Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcna1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13

Plasmid

#61087

Purpose

Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13, excluding exon 7, 11, 14-17

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Anti-Neuropeptide Y [L115/13R-2b]

Plasmid

#206639

Purpose

Mammalian Expression Plasmid of anti-Neuropeptide Y (Human). Derived from hybridoma L115/13-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuropeptide Y (Homo sapiens) recombinant Mouse monoclonal antibody (NPY Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFlox c-CTCF FKBPF36V_EYFP_p2aBsd

Plasmid

#163870

Purpose

Acute Depletion of CTCF-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Ctcf (Ctcf Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

Plasmid

#174634

Purpose

Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mCherry-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-122

Plasmid

#26325

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-122

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Expression

Mutation

dominant negative (Y699F)

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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GluN3A/NR3A glutamate receptor scFv [N416/57]

Plasmid

#206773

Purpose

Mammalian Expression of GluN3A/NR3A glutamate receptor scFV. Derived from hybridoma N416/57 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN3A/NR3A glutamate receptor (Mus musculus) recombinant scFV (Grin3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/90]

Plasmid

#206763

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/36]

Plasmid

#190563

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTG-mTDG-K341R

Plasmid

#52267

Purpose

Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV MEKK3 PB1

Plasmid

#12185

Depositor

Gary Johnson

Article

Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

Insert

MEKK3 (Map3k3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

PB1 domain: aa 36-127

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 p300 gRNA

Plasmid

#165591

Purpose

Insertion of p300 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Smarca2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-Nav1 Na+ channel [N419/40R]

Plasmid

#114552

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav1 Na+ channel (Human). Derived from hybridoma N419/40.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav1 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN5A Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Talin-C-18

Plasmid

#62763

Purpose

Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

Depositor

Michael Davidson

Insert

Talin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX Txk-SH2

Plasmid

#46526

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Txk (Txk Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 138-258

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Use

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307 GFP-Kif26b-C

Plasmid

#118483

Purpose

lentiviral expression of GFP-Kif26b-C in mammalian cells

Depositor

Henry Ho

Article

Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

Insert

mouse Kif26b-C (Kif26b Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Encodes GFP fused to amino acid 1735 to 2112 of m…

Promoter

EF1A

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Cdh1

Plasmid

#49776

Purpose

pENTR vector for mouse E-cadherin

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Cdh1 (Cdh1 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

Plasmid

#75129

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lox-stop-Lox p53 R270H

Plasmid

#14853

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R270H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Contact mutant R270H. (Murine p53 codon 270 corre…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
mApple-Talin-C-18

Plasmid

#62738

Purpose

Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:568, Emission:592

Depositor

Michael Davidson

Insert

Talin

Use

Tags

mApple

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
mApple-Profilin-10

Plasmid

#54940

Purpose

Localization: Cytoskeleton, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Profilin

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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