We narrowed to 13,645 results for: NTS

1. pMOD_C3012

   Plasmid

   #91099

   Purpose

   Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   PvUbi1

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGE656

   Plasmid

   #153254

   Purpose

   M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VRER-ABEmax

   Plasmid

   #119812

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAGM51559

   Plasmid

   #153226

   Purpose

   Control plasmid for knockout of TRY and CPC genes

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Cas9, guide RNA for AP3, FAST marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK364.306

   Plasmid

   #192642

   Purpose

   Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   J306 scRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEE392

   Plasmid

   #149282

   Purpose

   T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_mFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pKLV-EF1aGFP-W

   Plasmid

   #159291

   Purpose

   Lentiviral vector expressing GFP driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tetO-Myocd

   Plasmid

   #46033

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   Myocd (Myocd Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-SUN1_785

   Plasmid

   #125852

   Purpose

   Expresses SUN1_785 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_785 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

    Plasmid

    #200636

    Purpose

    Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPBpuro-COX2-EBFP2

    Plasmid

    #184720

    Purpose

    Exogenous COX2

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    COX2-EBFP2 (PTGS2 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE474

    Plasmid

    #153265

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE475

    Plasmid

    #153266

    Purpose

    M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p35S-A3A-PBE

    Plasmid

    #119773

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_A1510

    Plasmid

    #91036

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CP1041-CBEmax

    Plasmid

    #119803

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1041) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CeNL-Clathrin_pEGFP

    Plasmid

    #89540

    Purpose

    Cyan color variant of bright luminescent protein, fused to clathrin light chain

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    Clathrin light chain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMOD_A1910

    Plasmid

    #91041

    Purpose

    Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2-P2A-TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CP1249-ABEmax

    Plasmid

    #119809

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Use

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Use

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSUPER IKKe

    Plasmid

    #26211

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK epsilon (IKBKE Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3 HA Sam68 deltaKH

    Plasmid

    #17688

    Depositor

    David Shalloway

    Article

    Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

    Insert

    Sam68 (Khdrbs1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 157-256 deleted (KH domain)

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-HA-ERK2 D319N

    Plasmid

    #8979

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D319N

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

32. HBT_pcoCASphi_version2

    Plasmid

    #197932

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    2xNLS-2xFLAG

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-DUET-1-mRhubarb720-HO

    Plasmid

    #141201

    Purpose

    Expresses mRhubarb720 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb720
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, L196Q, T202D, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRU53

    Plasmid

    #167679

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMOD_A0502

    Plasmid

    #91012

    Purpose

    Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtUbi10

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS406-PHO5-GFP-hFAPP1 PH domain

    Plasmid

    #58723

    Purpose

    Expresses GFP-Tagged human FAPP1 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

37. Puro-iDEST

    Plasmid

    #75336

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    rice U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMOD_C3006

    Plasmid

    #91096

    Purpose

    Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    FMV 34S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge