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Plasmid#196877PurposeNeuron-specific expression of mOrange2 reporterDepositorInsertmOrange2
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-td-mCherry-CI
Plasmid#196851PurposeExpression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoterDepositorInserttd-mCherry
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG
Plasmid#185347PurposeMembrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tagDepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_139
Plasmid#180565PurposeSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2DepositorInsertSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin-S796A
Plasmid#186434PurposeExpresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
UseTagsmCherryExpressionMammalianMutationChanged Serine 796 to AlaninePromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_113
Plasmid#180540PurposeEntry vector containing Signal peptide region of CsgA including SecB-N22 sequencedDepositorInsertSignal peptide region of CsgA including SecB-N22 sequenced
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251_ Ptrc.x.tetO2::D-HicDH
Plasmid#185456PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.DepositorInsertD-HicDH from Lactobacillus paracasei DSM 20008
UseTagsExpressionBacterialMutationPromoterPtrc.x.tetO2Available sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_095
Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
iDianiSnFR
Plasmid#177741PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiDianiSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG660 lyn11-pFAST
Plasmid#172863Purposeexpresses lyn11-pFAST in mammalian cells (inner membrane labeling)DepositorInsertLyn11-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2
Plasmid#175751PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A
Plasmid#175752PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50ADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationY49A L50APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
iCytSnFR
Plasmid#177738PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCytSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_BrEt_SnFR
Plasmid#177740PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_BrEt_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_F_SnFR
Plasmid#177739PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_F_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TTBG
Plasmid#115232PurposeLentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2DepositorInsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TTBL
Plasmid#115233PurposeLentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2DepositorInsertmTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-Flpo
Plasmid#174378Purposealso known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinaseDepositorHas ServiceAAV RetrogradeInsertFLPo recombinase
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]
Plasmid#153542PurposeAAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]
Plasmid#153541PurposeAAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Topo-Tbxas1_ISH_S
Plasmid#170307PurposeEncoding partial sense strand of the mouse Tbxas1 gene for in situ hybridization.DepositorInsertPartial sense strand of the mouse Tbxas1 gene for in situ hybridization
UseFor ivfTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Topo-Tbxas1_ISH_AS
Plasmid#170308PurposeEncoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridizationDepositorInsertPartial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
UseFor ivfTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
LR (hsp70I: cPLA2-mK2)
Plasmid#162564PurposeMeasuring cPLA2 activation in live cells after heat shock.DepositorUseGateway donor vectorTagsmK2ExpressionMutationPromotercmlc2Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeT-GFP]
Plasmid#153539PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Oct4deltaPE-53BP1mCherry
Plasmid#141122PurposeConstruct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cellsDepositorInsertFragment of human 53BP1 (TP53BP1 Human)
UseMouse TargetingTagsmCherryExpressionMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP3
Plasmid#129506PurposeExpresses AvicFP3 constitutively in E. coli (most strains)DepositorInsertAvicFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [dRING]
Plasmid#126549PurposeExpresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationRING domain deletion mutant [dRING mutation is a …PromoterLTRAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [Y252D]
Plasmid#126550PurposeExpresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationY252D point mutantPromoterLTRAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-LM_lacO-cP-AOX1-sAR
Plasmid#126745Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-ICL1-LM_lacO-cP-AOX1-sAR
Plasmid#126744Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterICL1Available sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3038
Plasmid#122545PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_glo-1::CemOrange2 (glo-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
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GAL-p300-1901-2414
Plasmid#89103Purposemammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domainDepositorInsertp300 (EP300 Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 1901-2414PromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only