Addgene

We narrowed to 28,276 results for: REP

Showing: 3481 - 3510 of 28276 results

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pXJ40 GADD34 (514-674aa)

Plasmid

#75480

Purpose

mammalian expression of GADD34 514-674 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 514-674

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pD765 HMGCR 3'UTR

Plasmid

#105766

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

HMGCR 3'UTR (HMGCR Human)

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-mWasabi-G4

Plasmid

#164168

Purpose

Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xMyc-ERK2(K52R)

Plasmid

#129228

Purpose

Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

ERK2(K52R) (Mapk1 Rat)

Use

Tags

GST and Myc

Expression

Bacterial

Mutation

Changed Lysine 52 to Arginine

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (1-513aa)

Plasmid

#75479

Purpose

mammalian expression of GADD34 1-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 1-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pXJ40 GADD34 (214-513aa)

Plasmid

#78095

Purpose

mammalian expression of GADD34 214-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 214-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pSN23

Plasmid

#100108

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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ptCMV-153/47-VR-NG

Plasmid

#50704

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-GAL4-MiniWhite

Plasmid

#165890

Purpose

Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-F63Q

Plasmid

#104363

Purpose

Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acid: F63Q

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2

Plasmid

#82440

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Use

Tags

Expression

Mutation

The complementary region for GL2 siRNA control wa…

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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PWPXLD-GFP-Numb-S276D

Plasmid

#133752

Purpose

Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

NUMB endocytic adaptor protein (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

serine 276 converted to aspartic acid

Promoter

EF1-alpha

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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p63RhoGEF580-mVenus-N1

Plasmid

#84338

Purpose

Expresses p63 (580 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (580 aa) (ARHGEF25 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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epiCRISPRe

Plasmid

#135962

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only
pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC myc-mRAP1 I312R

Plasmid

#73137

Purpose

Expression of myc-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

isoleucine changed to an arginine at amino acid 3…

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RlACR_477-EYFP

Plasmid

#103771

Purpose

Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_477

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Use

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_AKAR5’

Plasmid

#114141

Purpose

Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5'

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO pGK

Plasmid

#68452

Purpose

TOPO construct expressing pGK for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Phosphoglycerate Kinase 1 Promoter (PGK1 Human)

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN24

Plasmid

#100109

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

Plasmid

#114731

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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