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We narrowed to 32,226 results for: Eng

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Showing: 5801 - 5850 of 32226 results

  7. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  11. pAC147-pCR8-dCas9VP64

    Plasmid
    #48219
    Purpose
    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP64 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP64
    Mutation
    D10A;H840A
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48219-plasmid-map-sequence-id-69504

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  18. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79885-plasmid-map-sequence-id-150051

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  19. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  24. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  33. GFP-Tac-TC

    Plasmid
    #162492
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162492-plasmid-map-sequence-id-323470

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  34. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP96
    Mutation
    D10A;H840A
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

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  44. pSmBtA3-d7F

    Plasmid
    #245784
    Purpose
    Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells
    Depositor
    Vsevolod Gurevich
    Article
    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3
    Insert
    arrestin-3 (ARR3 Bovine)
    Tags
    Flag and SmBiT
    Expression
    Mammalian
    Mutation
    deleted residues 175, 177, 178, 182-185
    Promoter
    CMV
    Available Since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    245784-plasmid-map-sequence-id-488534

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  47. pFB-D3-ASK1

    Plasmid
    #226619
    Purpose
    Co-expression of D3 and ASK1 complex in insect cells
    Depositor
    Ning Zheng
    Article
    Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:
    Inserts
    D3
    ASK1 (SKP1 Mustard Weed)
    Tags
    His tag followed with three copies of Msyb
    Expression
    Insect
    Mutation
    The flexible loop in D3 (Q478-D515) can be remove…
    Promoter
    Polyhedrin
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226619-plasmid-map-sequence-id-449175

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Showing: 5801 - 5850 of 32226 results