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We narrowed to 18,759 results for: nls

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Showing: 4681 - 4720 of 18759 results

  1. pCSN068

    Plasmid
    #101749
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101749-plasmid-map-sequence-id-202306

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  3. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Publication
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101920-plasmid-map-sequence-id-194554

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  4. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90086-plasmid-map-sequence-id-182687

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  7. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Publication
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  8. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50579-plasmid-map-sequence-id-75626

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  16. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid
    #247262
    Purpose
    Express ISCro4 recombinase and bridge RNA
    Depositor
    Patrick Hsu
    Publication
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    Expression
    Mammalian
    Mutation
    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…
    Available since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247262-plasmid-map-sequence-id-490480

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  21. pGY577

    Plasmid
    #166663
    Purpose
    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination
    Depositor
    Gaël Yvert
    Publication
    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:
    Insert
    LiCre
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    Cre: E340A, D341A
    Promoter
    CMV
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    166663-plasmid-map-sequence-id-330243

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  24. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

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  30. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90519-plasmid-map-sequence-id-287208

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  34. WN10151

    Plasmid
    #80441
    Purpose
    Expresses human codon-optimized dead (832A) LbCpf1
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead (D832A) LbCpf1
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80441-plasmid-map-sequence-id-151446

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  35. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Publication
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-dCas9VP192-T2A-EGFP
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69534-plasmid-map-sequence-id-122831

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  38. pEGFPN1-N-gamma-tubulin (1-333)

    Plasmid
    #87858
    Purpose
    Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS
    Depositor
    Maria Alvarado-Kristensson
    Publication
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gamma-tubulin 1 (TUBG1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Fragment from aa 1 to 333
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    87858-plasmid-map-sequence-id-178340

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  39. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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Showing: 4681 - 4720 of 18759 results