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Plasmid#131167PurposePCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSM002
Plasmid#122936PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML043C (Marked)
UseTagsExpressionYeastMutationYML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATACPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM001
Plasmid#122935PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC005
Plasmid#122939PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML071C (Marked)
UseTagsExpressionYeastMutationYML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGCPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_207
Plasmid#122938PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML070W (Marked)
UseTagsExpressionYeastMutationYML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_243
Plasmid#122940PurposeExpression of the marked mRNA under the control of insertional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoterPyml029w-tetO(2)Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM004
Plasmid#122937PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML065W (Marked)
UseTagsExpressionYeastMutationYML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAACPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_228
Plasmid#122933PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR025C (Marked)
UseTagsExpressionYeastMutationYBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_229
Plasmid#122934PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR026C (Marked)
UseTagsExpressionYeastMutationYBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAGPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SD](315)
Plasmid#122074PurposeExpressing GFP-Atg13[3SD]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SA](315)
Plasmid#122073PurposeExpressing GFP-Atg13[3SA]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD3685
Plasmid#113391Purposealpha factor inducible Ty3DepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSS63
Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T22I
Plasmid#1110DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with threonine 22 changed to isoleucine; re…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G81S
Plasmid#1111DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 81 changed to serine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G313S
Plasmid#1114DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 313 changed to serine; regulat…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/E381K
Plasmid#1115DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glutamic acid 381 changed to lysine; r…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A587T
Plasmid#1116DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 587 changed to threonine; regu…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A41V
Plasmid#1117DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 41 changed to valine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSL3 - Brr2 del113 N1104L
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL7 - Brr2 del247 N1104L
Plasmid#90257Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 247 amino acids and N1104LPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRRL MND GFP
Plasmid#36247DepositorInsertGFP (eGFP )
UseLentiviralTagsExpressionMutationPromoterMNDAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
KBB364
Plasmid#185083PurposeRFA1 fused to GFP under control of GAL1 promoterDepositorInsertRFA1
UseTagsGFPExpressionYeastMutationPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDFBB-eGFP
Plasmid#32550DepositorInsertegfp (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ntH6-eGFP
Plasmid#32557DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH760-CEN-mCherry_v3
Plasmid#40598DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ptet-eGFP
Plasmid#32554DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterptetAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterpBADAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRRL WS1.6 GFP
Plasmid#36249DepositorInsertGFP (eGFP )
UseLentiviralTagsExpressionMutationPromoterWAS (human 1.6 kb)Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pDA125
Plasmid#74249PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTJK517
Plasmid#121474PurposeCEN TRP1 CNA1DepositorInsertCNA1
UseYeast cen vectorTagsExpressionMutationPromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJLI-SUP35 (delta)2-5
Plasmid#1138DepositorInsertsup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationlast four repeats of sup35 N domain deleted, but …PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only