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Showing: 551 - 600 of 1692 results

  1. pCDNA3_PKA-CαN (K29,30A)-mVenus

    Plasmid
    #168485
    Purpose
    Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168485-plasmid-map-sequence-id-332538

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  4. pSW554 - pOp5:SP-mVenus-HDEL

    Plasmid
    #116006
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    116006-plasmid-map-sequence-id-227518

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  6. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106190
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106190-plasmid-map-sequence-id-204216

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  13. pMP108 Nanog-Venus-2a-mCherry

    Plasmid
    #159743
    Purpose
    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.
    Depositor
    Sergi Regot
    Article
    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d
    Insert
    Nanog homeobox (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    Venus-2a-mCherry
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159743-plasmid-map-sequence-id-330621

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  14. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106185
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106185-plasmid-map-sequence-id-204585

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  16. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106191
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    106191-plasmid-map-sequence-id-214865

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  17. Venus-30aa-Noxa-pEGFP-C1

    Plasmid
    #166762
    Purpose
    To increase the length of the linker between Venus and Noxa proteins
    Depositor
    David Andrews
    Insert
    Noxa (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166762-plasmid-map-sequence-id-328844

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  19. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106184
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    106184-plasmid-map-sequence-id-214885

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  20. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  22. mVenus-P75ECD-VfAU1-LOV_458

    Plasmid
    #63634
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by mVenus, the extracellular and transmembrane domain of p75NTR and a restriction site
    Depositor
    Harald Janovjak
    Insert
    mVenus-P75ECD-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63634-plasmid-map-sequence-id-105955

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  23. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  25. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  30. pSLIK-Venus-TmiR-G13

    Plasmid
    #25741
    Purpose
    Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    G alpha 13 miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25741-plasmid-map-sequence-id-12066

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  34. pSLIK-Venus-TmiR-G12

    Plasmid
    #25740
    Purpose
    Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    G alpha 12 miR-shRNA (Gna12 Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25740-plasmid-map-sequence-id-12065

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  36. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid
    #236072
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236072-plasmid-map-sequence-id-472895

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  37. pT/UBC-mVenus-P2A-NRASG12V

    Plasmid
    #236074
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Ubc
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236074-plasmid-map-sequence-id-472894

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  38. pT/CAGGS-NRASG12V-IRES-mVenus

    Plasmid
    #236076
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236076-plasmid-map-sequence-id-472892

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  39. pT/PGK-mVenus-P2A-NRASG12V

    Plasmid
    #236073
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    PGK
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236073-plasmid-map-sequence-id-472890

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  41. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #177424
    Purpose
    To express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.
    Depositor
    David Andrews
    Insert
    Noxa, PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    177424-plasmid-map-sequence-id-348448

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  47. pcDNA3.1 NKX6-2(1-196)-Venus

    Plasmid
    #209182
    Purpose
    Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-196) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209182-plasmid-map-sequence-id-413578

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  50. pcDNA3.1 NKX6-2(1-188)-Venus

    Plasmid
    #203714
    Purpose
    SPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent protein
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-188) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 189-277
    Promoter
    CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203714-plasmid-map-sequence-id-401198

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Showing: 551 - 600 of 1692 results