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Showing: 551 - 600 of 2294 results

  3. pET28-iLOV-Rsn2

    Plasmid
    #61687
    Purpose
    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV
    Depositor
    Brian Smith
    Insert
    Ranaspumin-2
    Use
    Tags
    His and iLOV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61687-plasmid-map-sequence-id-259998

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  12. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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  22. pET28b-Ets1-ETS

    Plasmid
    #85735
    Purpose
    Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440
    Depositor
    Gregory Poon
    Article
    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16
    Insert
    DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
    Use
    Tags
    6xHis following thrombin cleavage site LVPR|G
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    85735-plasmid-map-sequence-id-173055

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  30. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

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  41. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  43. eGFP-Silicatein-pET28

    Plasmid
    #198012
    Purpose
    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification
    Depositor
    Bryan Berger
    Article
    Vigil et al Materials Advances 2, 2023
    Insert
    eGFP-silicatein
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198012-plasmid-map-sequence-id-388331

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Showing: 551 - 600 of 2294 results