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We narrowed to 14,123 results for: cas9

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Showing: 221 - 240 of 14123 results

  2. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  5. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  9. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  10. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  16. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  17. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  18. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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Showing: 221 - 240 of 14123 results