We narrowed to 13,922 results for: cas9

1. pL40C_PGKintron_Cas9_Green

   Plasmid

   #134966

   Purpose

   Lentiviral vector coding Cas9 and mNeonGreen

   Depositor

   Beat Bornhauser

   Article

   Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

   Insert

   Cas9-P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   hPGK promoter with beta-globin intron

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

   Plasmid

   #136660

   Purpose

   Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   ABE7.10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and hSt1Cas9

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAtCas9_3

   Plasmid

   #91158

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-M-G6

   Plasmid

   #73437

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor G6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPhygCAS9

   Plasmid

   #63555

   Purpose

   Expresses CAS9 in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
   

   Use

   Leishmania expression

   Tags

   flag

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_human_ADAR2_ccC

   Plasmid

   #158112

   Purpose

   deletion hADAR2

   Depositor

   Emanuela Felley-Bosco

   Article

   Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

   Insert

   ADAR2 (ADARB1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_Puro

   Plasmid

   #167871

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. e-ciCas9_pcDNA5

   Plasmid

   #100553

   Purpose

   Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   e-ciCas9
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9m2

   Plasmid

   #47314

   Purpose

   Cas9 D10A+H840A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m2
   

   Use

   CRISPR

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. evoCas9

    Plasmid

    #138558

    Purpose

    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    evoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. bbCas9pluspAAA

    Plasmid

    #82581

    Purpose

    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Insert

    Cas9
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HeFSpCas9

    Plasmid

    #92355

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_EBFP2

    Plasmid

    #167857

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAtCas9_1

    Plasmid

    #91156

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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