We narrowed to 24,482 results for: Spr

1. PX458_ZBTB49_iso1_2

   Plasmid

   #128251

   Purpose

   Encodes gRNA for 3' target of human ZBTB49_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB49_iso1 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX330_SSX2B

   Plasmid

   #128252

   Purpose

   Encodes gRNA for 3' target of human SSX2B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   SSX2B gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJA46

   Plasmid

   #59928

   Purpose

   gRNA for cleavage at rde-1(D801) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   rde-1(D801) gRNA
   

   Use

   CRISPR

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJA45

   Plasmid

   #59927

   Purpose

   gRNA for cleavage at rde-1(D718) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   rde-1(D718) gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   Tet ON

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-Cas9-Blast

   Plasmid

   #83481

   Purpose

   Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

   Depositor

   Mohan Babu

   Inserts

   SpCas9
   Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   Tight Tre and human phosphoglycerate kinase 1 pro…

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCB320

   Plasmid

   #89359

   Purpose

   3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   puromycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

    Plasmid

    #49771

    Purpose

    Level 1 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    35Sp::hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFD4d-U6-1:white1-U6-3:white1

    Plasmid

    #84005

    Purpose

    pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Inserts

    white sgRNA-1
    white sgRNA-1
    

    Use

    CRISPR

    Promoter

    U6:1, U6:3, and dU6-1; dU6-3

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMVP-Cas9FL-P2A-turboGFP

    Plasmid

    #80941

    Purpose

    Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9FL
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Promoter

    Chicken beta actin (CAG)

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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