We narrowed to 15,043 results for: NOL
-
Plasmid#13393DepositorInsertC/EBPbeta (Cebpb Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB6677
Plasmid#106155PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2
Plasmid#192412PurposeTo act as a 1-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS
Plasmid#192413PurposeTo act as a 1-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6nH1-HD10kb
Plasmid#172657PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD10kbA/pegRNA-HD10kbB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G08
Plasmid#67142PurposeGateway destination vector for cell-free expression with C-term mCherry myc-tag tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis-MYC and mCherryExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3A9cbetaTCR.HSV2.3
Plasmid#69594PurposeTCR beta chain genomic transgenic construct for generation of gBT-I miceDepositorInsertTCR beta VJ region from clone HSV2.3 (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
pSV(X) C/EBPalpha
Plasmid#13392DepositorInsertC/EBPalpha (Cebpa Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC1748
Plasmid#127982PurposeAMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK407 (BSD-P2A-mAID-Nluc)
Plasmid#121197PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-Nluc
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)
Plasmid#191151PurposeExpression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L72:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ptet-eGFP
Plasmid#32554DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterptetAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AT1R DRY-AAY
Plasmid#45759DepositorInsertAT1R DRY-AAY (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationchanged amino acids 125-126 from DR to AAPromoterCMVAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB6684
Plasmid#106154PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6631
Plasmid#106161PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTF3-AK (clone3)
Plasmid#21165DepositorInsertPOUF5F1 (genomic DNA) (POU5F1 Human)
UseCre/Lox; Homologous recombinationTagsExpressionMutationNeoR, EGFP, and SV40 polyA are between the two ge…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterpBADAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PDGFRB
Plasmid#67130PurposeStandard for cell-free expression benchmarking.DepositorInsertPDGFRB (PDGFRB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS_miR-146aSponge_mCherry_GFP
Plasmid#149718PurposeLentiviral vector expressing a miR-146a decoy spongeDepositorInsert14X miR-146a binding sites
UseLentiviralTagsExpressionMammalianMutationG to T mutation in position 75 of the insertPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DSCP-Gateway
Plasmid#71507PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#59
Plasmid#110929PurposeExpresses proteins pan-neuronally in C. elegans (entire nervous system)DepositorInsertsrab-3 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
IBMc233
Plasmid#161939PurposeCarries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M2S_AmpR
Plasmid#119154PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191184PurposeExpression of RPLP1 sensor in mammalian cellsDepositorInsertmCherry:RPLP1.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192396PurposeTo act as a 2-input state of the AND gate.DepositorInsertAct2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192391PurposeTo act as a 2-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1
Plasmid#62600PurposeConstitutive expression of FPR1 in mammalian cellsDepositorInsertFPR1 (FPR1 Human)
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASH3 [pmyo-3::BeCNG1-YFP]
Plasmid#168167PurposeExpression of BeCNG1-YFP in BWMs of C. elegansDepositorInsertBeCNG1-YFP
UseTagsYFPExpressionWormMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HaloLICNot
Plasmid#140185PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsHaloExpressionMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)
Plasmid#104117PurposesynOptopatch constructDepositorInsertQuasAr3-dark Citrine
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-HSV2.3 TCR beta chain
Plasmid#74077PurposegBT-I TCR beta chain cDNA in pCRIIDepositorInsertTCR beta cDNA clone HSV2.3 (Tcrb Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 SV40-AcrIIA4 (GB3333)
Plasmid#160605PurposeSV40-AcrIIA4 codon optimized for N.benthamianaDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACBB-TetT-LVA
Plasmid#32555DepositorInsertTetR
UseSynthetic BiologyTagsLVA tagExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
myc-Rapr-FAK-YM
Plasmid#25927DepositorInsertRapR-FAK (Ptk2 Mouse)
UseTagsMycExpressionMammalianMutationThis myc-RapR-FAK-YM plasmid contains FAK mutatio…PromoterAvailable sinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only