We narrowed to 8,363 results for: construct

1. mOtop2_pGEMHE

   Plasmid

   #114675

   Purpose

   This construct was used to express mouse Otop2 in Xenopus laevis oocytes

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 2 (Otop2 Mouse)
   

   Use

   For generating the mrna, which can highly express…

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTorPE-G-GECO1

   Plasmid

   #32466

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.0
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

   Expression

   Bacterial

   Mutation

   GCaMP3 K119I/L173Q/S404G/E430V

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPE1

   Plasmid

   #209981

   Purpose

   Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (pMB1_ampR_B0017)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLPE2

   Plasmid

   #209982

   Purpose

   Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (ampR_B0017)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLPE3

   Plasmid

   #209985

   Purpose

   Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (pMB1_cat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLPP5

   Plasmid

   #209967

   Purpose

   Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_sppQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pRepair-MPP8_mAID-P2A-BFP

   Plasmid

   #175036

   Purpose

   Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   mAID-P2A-BFP
   

   Use

   Hdr donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6_(GLuc)_INT[3xPP7_SL]

   Plasmid

   #68424

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLHDR02_EGFP_tCD8

   Plasmid

   #124061

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   EGFP
   tCD8
   

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Human UbC promoter modified

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry-C1-PsACR1

    Plasmid

    #85465

    Purpose

    Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct PsChR1 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. RVD-AA5

    Plasmid

    #173240

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RVD-AA9

    Plasmid

    #173244

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-mTagBFP-CAAX

    Plasmid

    #75176

    Purpose

    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-H2GH

    Plasmid

    #182202

    Purpose

    Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    histone2B-HaloTag-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

    Plasmid

    #159799

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C2-TetR-KRf-TetR

    Plasmid

    #216270

    Purpose

    Construct 3: TetR - Lipid binding domain KRφ - TetR

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    TetR - Lipid binding motif (KRf) - TetR
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173228

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173229

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NylB_pET29b

    Plasmid

    #215414

    Purpose

    Expression construct for NylB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

    Plasmid

    #104122

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    spine-jRGECO1a-P2A-QuasAr-dark Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pL0_AC-CEN_ARS_HIS

    Plasmid

    #202129

    Purpose

    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    CEN_ARS_HIS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ARS sequence mutated to remove sapI site for dome…

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pENTR-L3-Csy4-Cas9n-EGFP-L2

    Plasmid

    #141046

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9n-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pL0_EF-PtBle

    Plasmid

    #202180

    Purpose

    Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    ShBle expression cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pHD-3xP3-dsRed-DattP

    Plasmid

    #133559

    Purpose

    Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    PQS1

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

    Plasmid

    #104116

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23110

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-mGrn-myc

    Plasmid

    #178944

    Purpose

    Construct for expressing murine progranulin (Grn) in AAV

    Depositor

    Erik Roberson

    Article

    Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

    Insert

    Grn (Grn Mouse)
    

    Use

    AAV

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CMV-mEGFP-PKCg-C1

    Plasmid

    #112266

    Purpose

    ITRACKgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMH-SFB-RNF169

    Plasmid

    #99392

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

    Depositor

    Michael Huen

    Article

    Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3E-mCherry-NS-MCS

    Plasmid

    #75179

    Purpose

    mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mCherry-NS-MCS
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTol1-UAS:Chronos-tdTomato

    Plasmid

    #124232

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    Chronos-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23107

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pconst #1-RFP

    Plasmid

    #81075

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23119

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pL0_EF-H4

    Plasmid

    #202178

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pL0_AC-H4

    Plasmid

    #202122

    Purpose

    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pL0_AC-P49

    Plasmid

    #202120

    Purpose

    Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pME-mKOFP2-CAAX

    Plasmid

    #75155

    Purpose

    Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pKS913.CB18.31

    Plasmid

    #69569

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-1 mice

    Depositor

    Francis R. Carbone

    Article

    Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

    Insert

    TCR beta (Tcrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.1-deltaA1-VWF

    Plasmid

    #124823

    Purpose

    Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted A1 domain

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge