We narrowed to 119,113 results for: mpi

1. EF1a-mito-dsRED2

   Plasmid

   #174541

   Purpose

   mitochondria targeted dsRED expressed off of EF1a promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT643

   Plasmid

   #8610

   Depositor

   Robert Hartley

   Article

   Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

   Insert

   barstar A
   

   Expression

   Bacterial

   Mutation

   Two cysteines of barstar changed to alanine, C40A…

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. IDG_TMEM63A_OE_1

   Plasmid

   #161667

   Purpose

   Overexpresses TMEM63A

   Depositor

   Mike McManus

   Insert

   TMEM63A (TMEM63A Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Dec. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCLIP-tag (m) Vector

   Plasmid

   #101136

   Purpose

   Expression of protein of interest with CLIP-Tag in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   CLIP-tag (CLIP10)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

   Plasmid

   #107055

   Purpose

   Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLX-PGK-hCDK4

   Plasmid

   #114314

   Purpose

   Constitutive expression of the CDK4 protein

   Depositor

   Patrick Salmon

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS40H

   Plasmid

   #35479

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSII406

   Plasmid

   #35442

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1-IRES eGFP

   Plasmid

   #128059

   Purpose

   Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2RZ

    Plasmid

    #27664

    Depositor

    Graeme Conn

    Article

    Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Type

    Empty backbone

    Use

    Rna transcription

    Tags

    HDV Ribozyme

    Available Since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti- V6.3 Ultra

    Plasmid

    #106172

    Purpose

    lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable

    Depositor

    Ewa Snaar-Jagalska

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    T2A, P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTipi2.1

    Plasmid

    #225877

    Purpose

    Allows for expression of recombinant antibodies

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. SPOCK2_22-424_AviN_HisC

    Plasmid

    #195877

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SPOCK2 (SPOCK2 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET His6 Sumo TEV LIC cloning vector (2S-T)

    Plasmid

    #29711

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-C

    Plasmid

    #213496

    Purpose

    Encodes Node C of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    lambda cI 5G6GP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBM16

    Plasmid

    #45344

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. YEpADE1

    Plasmid

    #22495

    Depositor

    Beth Montelone

    Insert

    ADE1 (ADE1 Budding Yeast)
    

    Use

    Yeast shuttle

    Mutation

    Original 4067 bp HindIII-HindIII insertion (see Y…

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. repressilator (pZS1-lTlrLLtCL)

    Plasmid

    #26489

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    LacI, tetR
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid

    #191442

    Purpose

    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hROSA26 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. TAp63alpha-FLAG

    Plasmid

    #27008

    Depositor

    David Sidransky

    Insert

    TAp63alpha (TP63 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pR6K-GFP-CASTIF

    Plasmid

    #199653

    Purpose

    R6K plasmid encoding a type I-F CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-F systems
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pJO672 (pRS415 Gal1-Ufd4-flag)

    Plasmid

    #159590

    Purpose

    expresses Ufd4-flag in yeast cells

    Depositor

    Alexander Varshavsky

    Insert

    Ufd4
    

    Expression

    Yeast

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pNBU2_repA1

    Plasmid

    #211910

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBabe bleo human PPAR gamma 2

    Plasmid

    #11439

    Depositor

    Ronald Kahn

    Insert

    PPAR gamma 2 (PPARG Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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30. SPLICSFAST-LDh-MT-P2A

    Plasmid

    #214896

    Purpose

    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LDh-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. pCAG-N2cG

    Plasmid

    #100811

    Purpose

    express rabies virus CVS N2c strain glycoprotein gene (N2cG)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain glycoprotein gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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32. PB CAG-sfGFP-T2A-iCreERT2

    Plasmid

    #177309

    Purpose

    CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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33. CX3CR1-EGFP

    Plasmid

    #24061

    Depositor

    Dan Littman

    Insert

    CX3CR1 (Cx3cr1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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34. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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35. pcDNA3.1-Clover-mRuby2

    Plasmid

    #49089

    Purpose

    Clover-mRuby2 tandem fusion for FRET calibration

    Depositor

    Kurt Beam

    Insert

    Clover-mRuby2 fusion
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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36. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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37. pcDNA3.1-Nluc

    Plasmid

    #180501

    Purpose

    Mammalian expression of Nluc

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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38. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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39. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid

    #219828

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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40. mFGF2

    Plasmid

    #22084

    Depositor

    Gail Martin

    Insert

    FGF2 (Fgf2 Mouse)
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

41. pLenti6MFV SLC25A4

    Plasmid

    #194133

    Purpose

    Expresses ANT1 (SLC25A4) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC25A4 (SLC25A4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. GFAP-Flex-MCS-WPRE-SV40 - GCH32

    Plasmid

    #230830

    Purpose

    AAV cloning vector for CRE-dependent expression under the GFAP promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLVX-EGFP-C3-cenexin

    Plasmid

    #73334

    Purpose

    Lentiviral vector encoding cenexin with an N-terminal EGFP tag

    Depositor

    Manuel Thery

    Insert

    cenexin/ODF2 (ODF2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET15-MHL

    Plasmid

    #26092

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. KP.3

    Plasmid

    #233340

    Purpose

    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (KP.3) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    F456L, Q493E, V1104L on the basis of JN.1

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. 1245 pCMVb p300

    Plasmid

    #10717

    Depositor

    William Sellers

    Insert

    p300 (EP300 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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48. pT2/SV-Venus-SIINFEKL

    Plasmid

    #111624

    Purpose

    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

    Depositor

    Jon Yewdell

    Insert

    Venus-LEQLE-SIINFEKL-TEW
    

    Tags

    LEQLE-SIINFEKL-TEW

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLenti6MFV SLC40A1

    Plasmid

    #194132

    Purpose

    Expresses MTP1 (SLC40A1) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC40A1 (SLC40A1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge