We narrowed to 50,496 results for: ang

1. pUAST td tomato Rab5 WT

   Plasmid

   #58446

   Purpose

   expression of drosophila td tomato Rab5

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   td tomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pM122: pAAV-EFS-CasRx

   Plasmid

   #166870

   Purpose

   AAV vector for expressing CasRx

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223_HcRed

   Plasmid

   #25892

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NAX_CAGGS_CAS9_m4_VPR_KanR neo

   Plasmid

   #167885

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Akt-Scramble

   Plasmid

   #175014

   Purpose

   Negative-control construct for Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-Scramble
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmiR-report-hVEGFA

   Plasmid

   #112553

   Purpose

   3'UTR reporter assay

   Depositor

   Shusheng Wang

   Article

   Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-Flp-DOG-NW

   Plasmid

   #75469

   Purpose

   Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Has Service

   AAV1

   Insert

   Flp-DOG
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Hy_pMT Flag-UAP56 WT

    Plasmid

    #110125

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. S1-Fc

    Plasmid

    #164220

    Purpose

    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike S1 domain (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-H2A-Akt-STOPS

    Plasmid

    #175010

    Purpose

    Akt-STOPS targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-mCherry-Akt-STOPS
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRetroSuper-shSrc

    Plasmid

    #26982

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Src (SRC Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LRP6-m5-pCS2-VSVG

    Plasmid

    #27283

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    Changed S/T of all five PPP(S/T)P motifs to Alani…

    Promoter

    Available Since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pQE-30-RexYFP

    Plasmid

    #141070

    Purpose

    Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    RexYFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed Leucine 169 to Proline, changed Tyrosine …

    Promoter

    T5

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYJ34-PB-CRISPR-BANCR-E1-gRNA

    Plasmid

    #131077

    Purpose

    long non-coding RNA BANCR knock out

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Insert

    BANCR Exon1 gRNA (BANCR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroSuper-shFyn

    Plasmid

    #26985

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Fyn (FYN Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-Lyn-Akt-STOPS

    Plasmid

    #175009

    Purpose

    Plasma membrane-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    Lyn-mCherry-Akt-STOPS
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pGP-AAV-CAG-iGABASnFR2n-WPRE

    Plasmid

    #218884

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-VHL W117R-pBabe-puro

    Plasmid

    #19239

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tryptophan 117 to Arginin

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCFJ150-Cas9(dpiRNA)

    Plasmid

    #107940

    Purpose

    eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    Cas9(dpiRNA)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eft-3

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CAG-miniSOG

    Plasmid

    #193913

    Purpose

    Control plasmid that expresses non-targeted miniSOG in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGP-AAV-GFAP-iGABASnFR2n-WPRE

    Plasmid

    #218872

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    GFAP

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pLKO.1-puro-PARG shRNA

    Plasmid

    #23260

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

    Insert

    poly(ADP-ribose) glycohydrolase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-Cdk9 T186A

    Plasmid

    #28103

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Hy_pMT dati

    Plasmid

    #91800

    Purpose

    Expresses the Drosophila dati circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    dati (dati Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-HA-mIFITM3-C71,72,105A

    Plasmid

    #58396

    Purpose

    Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 71, 72, and 105 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

43. NAX_CAGGS_CAS9_m4_KanR neo

    Plasmid

    #167869

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRW006-SM-destination-pro35S-Cas9

    Plasmid

    #104439

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3-Lyn-AktAR2

    Plasmid

    #175013

    Purpose

    Plasma membrane-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    Lyn-mCherry-Akt-STOPS
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. HA-VHL Y98N-pBabe-puro

    Plasmid

    #19236

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Asparagin

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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50. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Promoter

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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