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Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-mClover3-CD4-bla
Plasmid#179441PurposeDonor vector to knock in mClover3 C-terminal to human CRY1 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-Twin-Strep
Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-LaminB1-10
Plasmid#56165PurposeLocalization: Nuclear Envelope, Excitation: 604, Emission: 659DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Flag-RNF168 delta RING
Plasmid#133982Purposemammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of RINGPromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBactin-EGFP-NEDD1-gTBD
Plasmid#196857PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertEGFP-N-gTBD (NEDD1 Human)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Actin-C-18
Plasmid#56151PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorInsertNM_001101.3 (ACTB Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFRT-TO-RPB1-His K1278R CRres si2,4R
Plasmid#139406PurposeDox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorInsertRPB1 (POLR2A Human)
UseTags6xHisExpressionMammalianMutationK1278RPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-ATG
Plasmid#153429PurposeExpresses a gRNA that overlaps the startcodon of human CTNNB1DepositorInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-MCAK-C-7
Plasmid#56168PurposeLocalization: Microtubules/Kinesin, Excitation: 604, Emission: 659DepositorInsertMCAK (KIF2C Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEX-YFP-STIM1-deltaK
Plasmid#18861DepositorInsertSTIM1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationStop codon inserted at amino acid postion 671 of …PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-Halo-CD4-bla
Plasmid#179445PurposeDonor vector to knock in Halotag C-terminal to human CRY1 geneDepositorInsertHaloTag (Halo7 Synthetic)
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 4NQ
Plasmid#121456Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1
Plasmid#135422PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFV2-mVenus
Plasmid#185653PurposeNDUFV2 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFV2 (NDUFV2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1s
Plasmid#135420PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variantDepositorHas ServiceAAV1InsertjYCaMP1s
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-Rosmarinus
Plasmid#183462PurposeTo produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion proteinDepositorInsertAlb (Alb Mouse)
UseAAVTagsRosmarinusExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFS6-mVenus
Plasmid#185806PurposeNDUFS6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFS6 (NDUFS6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SYFP2-CTNNB1
Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
UseTagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-mCarmine
Plasmid#183464PurposeTo produce AAV with the P3 promoter to express Alb-mCarmine fusion proteinDepositorInsertAlb (Alb Mouse)
UseAAVTagsmCarmineExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA7-mVenus
Plasmid#185647PurposeNDUFA7 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA7 (NDUFA7 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1
Plasmid#133978Purposemammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P-1-HRD1 aa 236-444
Plasmid#185349PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorInsertHRD1/SYVN (SYVN1 Human)
UseTagsGSTExpressionBacterialMutationaa 236-444 onlyPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63887PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1/MIU2
Plasmid#133981Purposemammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDB-hPER2-Halo-CD4-bla
Plasmid#179451PurposeDonor vector to knock in Halotag C-terminal to human PER2 geneDepositorInsertHaloTag (Halo7 Synthetic)
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn iCyt_BrEt_SnFR_PM
Plasmid#177757PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpAAV9-hSyn iCyt_BrEt_SnFR_PM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 365-513
Plasmid#78796PurposeTo overexpress TIP60 365-513 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutationaa365-513PromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Calnexin-N-14
Plasmid#56154PurposeLocalization: Endoplasmic Reticulum, Excitation: 604, Emission: 659DepositorInsertCalnexin (CANX Human)
UseTagsmCardinalExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Desmoplakin II tension sensor (FL-based)
Plasmid#118721PurposeThe FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-Luciferase-hvTK-bla
Plasmid#179444PurposeDonor vector to knock in firefly Luciferase C-terminal to human CRY1 geneDepositorInsertLuciferase
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN
Plasmid#69582PurposeExpresses p53 tagged with mKate2 and split N-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU2
Plasmid#133979Purposemammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUSEss-CHIg-mG3_CH3-1
Plasmid#105859PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH3 derived…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2-1
Plasmid#105857PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH2 derived…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only