We narrowed to 28,899 results for: PLE

1. pgRNA_hMECP2_promoter_No6

   Plasmid

   #194900

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.6
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA_hMECP2_promoter_No7

   Plasmid

   #194901

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.7
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pA-RFP-rG2

   Plasmid

   #188969

   Purpose

   IPTG inducible mCherry with sgRNA

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   mCherry
   sgRNA: agtccatgtaatcagcgtctactagt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLiP-G2

   Plasmid

   #188964

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-RanGAP

   Plasmid

   #175235

   Purpose

   Bacterial expression and purification, high affinity SUMOylation substrate

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_ReBphP-PCM_IRES_sfGFP

   Plasmid

   #166917

   Purpose

   Express ReBphP-PCM and sfGFP

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   ReBphP-PCM
   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSWD95Y14D

   Plasmid

   #159098

   Purpose

   CckA FRET Sensor with cyclic-di-GMP point mutant

   Depositor

   Seth Childers

   Article

   Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

   Insert

   CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutated Y514 to D514

   Promoter

   Xylose

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

   Plasmid

   #140223

   Purpose

   Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   PGK

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. K8.1Pr-pGL4.16-OriLyt

   Plasmid

   #131038

   Purpose

   Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

   Depositor

   Britt Glaunsinger

   Article

   Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

   Insert

   KSHV Lytic Origin of replication
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXW109ABS62(RS)

    Plasmid

    #123153

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS62, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXW109ABS67(RS)

    Plasmid

    #123154

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109. ParsR-ABS67, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRPL28(H39L) SpHIS5 (pNTI665)

    Plasmid

    #115435

    Purpose

    Expresses H39L RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H39L; MfeI site in intron

    Promoter

    ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCML 314

    Plasmid

    #98584

    Purpose

    pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Inserts

    CsrA
    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-6NBC-GFP-PGK-Puro-Barcode81

    Plasmid

    #83758

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode81
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    deletion of T in starting ATG of GFP (please see …

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-6NBC-GFP-PGK-Puro-Barcode71

    Plasmid

    #83748

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode71
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    deletion of AA5-233 in GFP (please see depositor …

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVH009

    Plasmid

    #80399

    Purpose

    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain and N-terminal 3xFLAG tag, …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-myc-mCE NLS

    Plasmid

    #82465

    Purpose

    Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids Nuclear Localization Signal

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-myc NES-mCE

    Plasmid

    #82463

    Purpose

    Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-myc-mCE(211-597)

    Plasmid

    #82462

    Purpose

    Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE(211-597) (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p11d-tRPA(123)

    Plasmid

    #102613

    Purpose

    Expression of human RPA in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Inserts

    RPA1 (RPA1 Human)
    RPA2 (RPA2 Human)
    RPA3 (RPA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. [#GS16-LV] Syn1-MitoTRACER - Donor

    Plasmid

    #233565

    Purpose

    Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKSE401

    Plasmid

    #62202

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge